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SENSISpec Ovalbumin ELISA Kit 48-Well Allergen Testing

Fiyat Sorunuz

High-sensitivity enzyme immunoassay for ovalbumin detection and quantification in food and beverage matrices

  • Sandwich enzyme immunological assay principle
  • Detection at 450 nm using HRP-TMB substrate system
  • Lower limit of detection: 4 ppb
  • Quantitative range: 25–500 ppb
  • Multi-point calibration with 5 standard levels
  • 48-well microtiter plate format for high-throughput analysis
  • Streamlined 60-minute total incubation protocol

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The SENSISpec Ovalbumin ELISA Kit represents a highly sensitive detection system for ovalbumin quantification, particularly suited for determining ovalbumin residues in wine and other food products. Chicken egg white (Gallus gallus) proteins constitute a significant food allergen source. While yolk proteins possess minimal allergenic properties, egg white contains multiple allergenic proteins. Ovalbumin, together with lysozyme, ovomucoid, ovotransferrin, and livetin, comprises the primary allergenic fraction of egg white. Both egg powder and purified ovalbumin are frequently employed as clarifying agents in winemaking. For allergic individuals, ovalbumin consumption represents a critical concern, as even trace amounts of allergen can trigger reactions ranging from mild symptoms to anaphylactic shock in severe cases.

Technical Specifications

Stock Code HU0030041
Test Principle Sandwich enzyme immunological assay
Format 48-well Microtiter plate
Total Incubation Time 60 minutes (20/20/20 minutes)
Calibration Standards 0; 25; 100; 250; 500 ppb
Detection Wavelength 450 nm (HRP + TMB)
Limit of Detection (LOD) 4 ppb
Quantitation Range 25–500 ppb
Storage Temperature 2–8°C

Applications

  • Wine and beverage allergen residue monitoring
  • Food manufacturing quality control and allergen verification
  • Regulatory compliance testing for ovalbumin content
  • Allergy risk assessment in food processing facilities
  • Clarity agent residue verification in beverage production
  • Hypoallergenic product certification

Frequently Asked Questions

What is the principle of this ELISA kit?
This kit employs a sandwich enzyme immunoassay principle, where ovalbumin is captured by immobilized antibodies and detected using enzyme-conjugated detection antibodies with HRP-TMB chromogenic substrate.
What is the detection sensitivity?
The assay provides a limit of detection (LOD) of 4 ppb with a reliable quantitative range of 25–500 ppb, utilizing a 5-point calibration curve.
Why is ovalbumin testing important?
Ovalbumin is a major egg white allergen. Its presence in food products poses serious health risks for allergic individuals, potentially causing reactions from mild symptoms to anaphylaxis. Accurate quantification ensures product safety and regulatory compliance.
Is this suitable for wine testing?
Yes, the kit is specifically optimized for quantifying ovalbumin residues in wine and other beverage matrices, as ovalbumin is commonly used as a clarification/fining agent in winemaking.
What is the total assay time?
The complete protocol requires 60 minutes of incubation time, divided into three 20-minute sequential steps, providing rapid turnaround for high-throughput analysis.