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Hazelnut ELISA Kit SENSISpec 48 Wells Allergen Test

Fiyat Sorunuz

High-sensitivity sandwich ELISA for quantitative detection of hazelnut residues in food matrices. 48-well microplate format with 0.07 ppm LOD.

  • Sandwich enzyme immunoassay principle for enhanced specificity
  • Detection limit (LOD): 0.07 ppm for reliable trace analysis
  • Quantification range: 1-40 ppm covering critical safety thresholds
  • Total incubation time: 60 minutes (20/20/20 minute intervals)
  • Five-point calibration curve (0, 1, 4, 10, 40 ppm standards)
  • HRP-TMB detection at 450 nm wavelength
  • 18-month shelf life at 2-8°C storage temperature
  • Microplate format for 48 simultaneous sample analysis

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SENSISpec Hazelnut ELISA Kit represents a highly sensitive detection system specifically designed for quantifying hazelnut residues in cookies, cereals, ice cream, and chocolate products. Hazelnut protein fraction constitutes approximately 13% of total hazelnut content, with numerous proteins documented as allergenic, including Cor a 9 and Cor a 11. These allergenic proteins exhibit high thermal stability and resistance to various manufacturing processes, making hazelnut a critical food allergen. For individuals with hazelnut allergy, hidden hazelnut allergens in processed foods present significant health risks. Even trace quantities can trigger allergic reactions and anaphylactic shock in severe cases. Cross-contamination during manufacturing processes is frequently encountered, particularly in chocolate, confectionery, and grain processing facilities. This kit enables reliable allergen detection to ensure food safety and regulatory compliance.

Technical Specifications

Stock Code HU0030034
Test Principle Sandwich enzyme immunological assay
Format Microtiter plate
Well Capacity 48 wells
Incubation Time 60 minutes (20/20/20 minutes)
Calibration Standards 0; 1; 4; 10; 40 ppm
Detection Wavelength 450 nm (HRP + TMB)
Limit of Detection (LOD) 0.07 ppm
Quantification Range 1 – 40 ppm
Shelf Life 18 months
Storage Temperature 2–8°C

Applications

  • Allergen residue monitoring in chocolate and confectionery manufacturing
  • Hazelnut contamination detection in grain products and cereals
  • Quality control testing in ice cream and frozen dessert production
  • Cross-contamination surveillance in multi-allergen processing facilities
  • Regulatory compliance testing for food safety standards
  • Finished product authentication and allergen declaration verification
  • Supplier raw material testing and ingredient verification

Frequently Asked Questions

What allergen proteins does this kit detect?
The SENSISpec Hazelnut ELISA kit is designed to detect hazelnut allergen proteins, particularly Cor a 9 and Cor a 11, which are thermostable and resistant to food processing conditions.
What is the minimum detectable hazelnut concentration?
The Limit of Detection (LOD) is 0.07 ppm, enabling sensitive detection of trace hazelnut contamination in processed foods.
What samples can be tested?
The kit is suitable for testing cookies, cereals, ice cream, chocolate, confectionery, and other processed food products that may contain hazelnut residues.
How long does the complete assay take?
Total assay time is 60 minutes, consisting of three 20-minute incubation steps with washing intervals.
What storage conditions are required?
The kit must be stored at 2–8°C (refrigerated conditions) and maintains stability for 18 months under proper storage.
Can this kit detect hazelnut in cross-contaminated samples?
Yes, with a LOD of 0.07 ppm, the kit can reliably detect cross-contamination levels, which is critical for cross-allergen manufacturing environments.