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SENSISpec Mustard ELISA Kit 96 Wells – Allergen Testing

Fiyat Sorunuz

High-sensitivity sandwich enzyme immunoassay for mustard protein detection in food matrices

  • Sandwich enzyme immunoassay format for robust allergen detection
  • 96-well microplate format enabling high-throughput analysis
  • Detection limit (LOD): 1 ppm for enhanced sensitivity
  • Quantitation range: 2–60 ppm covering regulatory thresholds
  • High cross-reactivity: 50% (black mustard), 59% (brown mustard), 48% (charlock mustard)
  • Validated on automated ELISA platforms (The BoltTM system)
  • Heat-stable allergen detection suitable for processed foods
  • 24-month shelf life with standard 2–8°C storage

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The SENSISpec Mustard ELISA kit delivers highly sensitive detection of yellow mustard (Sinapis alba) and demonstrates excellent cross-reactivity for brown mustard (Brassica juncea) and black mustard (Brassica nigra). The assay is optimized for quantifying mustard residues in processed foods including sausages, sauces, soups, cheese, and spice blends. Mustard seeds contain approximately 30-35% protein fraction, with allergens such as Sin a 1 and Bra j 1 being heat-stable and resistant to various food processing conditions. The kit has been validated on The BoltTM automated ELISA system and provides reliable allergen quantitation across multiple food categories.

Technical Specifications

Stock Code HU0030016
Assay Principle Sandwich enzyme immunoassay (EIA)
Format 96-well microtiter plate
Incubation Time 60 minutes (20/20/20 minutes)
Standard Concentrations 0; 2, 6; 20; 60 ppm
Detection Wavelength 450 nm (HRP + TMB substrate)
Limit of Detection (LOD) 1 ppm
Quantitation Range 2–60 ppm
Cross-Reactivity Black mustard 50%, Brown mustard 59%, Charlock mustard 48%, Coriander 0.0006%, Canola 59%
Shelf Life 24 months
Storage Conditions 2–8°C

Applications

  • Allergen detection in processed meat products (sausages, deli meats)
  • Mustard residue quantitation in sauces and condiments
  • Allergen screening in soup and broth preparations
  • Dairy product allergen analysis (cheese, butter blends)
  • Spice mixture and seasoning allergen verification
  • Regulatory compliance testing (EU labeling requirements)
  • Cross-contact risk assessment in manufacturing facilities
  • Quality assurance for food allergen control programs

Frequently Asked Questions

What is the minimum detection limit for this ELISA kit?
The limit of detection (LOD) is 1 ppm, enabling identification of trace amounts of mustard allergen in food samples.
Does this kit detect all mustard species?
The kit is optimized for yellow mustard detection with high cross-reactivity (≥50%) to black and brown mustard species due to protein homology.
Is the assay compatible with automated systems?
Yes, the kit has been validated on The BoltTM automated ELISA system for standardized, high-throughput allergen analysis.
How resistant is this assay to food processing?
Mustard allergens (Sin a 1, Bra j 1) are heat-stable proteins that remain detectable across various thermal and mechanical processing conditions.
What is the total assay runtime?
Total incubation time is 60 minutes (three sequential 20-minute incubation steps), plus additional time for plate preparation and substrate reaction.
What storage requirements apply to this kit?
Store at 2–8°C (refrigerated). The kit maintains stability for 24 months under recommended storage conditions.