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SENSISpec ELISA Lysozyme Kit 48 Well Allergen Test

Fiyat Sorunuz

Highly sensitive ELISA kit for detection and quantification of lysozyme residues in wine, cheese, and food products

  • Highly sensitive detection system for lysozyme residues in food matrices
  • Specific application for wine and cheese allergen screening
  • Low Limit of Detection (LOD) of 2 ppb
  • Wide quantitation range from 25–250 ppb
  • No cross-reactivity with other food allergens
  • Microtitre plate format with 48 wells
  • HRP + TMB chromogenic detection at 450 nm
  • 24-month shelf life when stored at 2–8°C

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SENSISpec Lysozyme ELISA kit represents a highly sensitive detection system for lysozyme, with the capability to determine lysozyme residue levels specifically in wine and cheese applications. Chicken egg (Gallus gallus) is protein-rich and represents an important food source for humans. While allergenic properties of egg yolk proteins are low, many proteins in egg white are known to be allergenic. In addition to ovalbumin, ovotransferrin, ovomucoid, and livetin, lysozyme represents an important allergen. It is primarily used as a preservative in the wine and cheese industries. Lysozyme consumption is a critical issue for allergic individuals. Even very low amounts of allergen can cause allergic reactions, and in severe cases can lead to anaphylactic shock. Non-disclosure of lysozyme addition to food may be dangerous for allergic individuals.

Technical Specifications

Stock Code HU0030036
Test Principle SENSISpec ELISA Lysozyme
Format Microtitre plate
Incubation Time 60 minutes (20/20/20 minutes)
Standards 0; 25, 50; 100; 250 ppb
Detection Wavelength 450 nm (HRP + TMB)
Limit of Detection (LOD) 2 ppb
Quantitation Range 25 – 250 ppb
Cross-reactivity None
Shelf Life 24 months
Storage Temperature 2–8°C

Applications

  • Detection and quantification of lysozyme residues in wine samples
  • Detection and quantification of lysozyme residues in cheese samples
  • Allergen screening in processed food products
  • Quality control and safety testing in food manufacturing
  • Compliance verification with allergen labeling regulations
  • Regulatory enforcement testing for undeclared allergens

Frequently Asked Questions

What is lysozyme and why is it considered a food allergen?
Lysozyme is a protein naturally found in egg white that is widely used as a preservative and antimicrobial agent in wine and cheese production. It is classified as a major food allergen that can trigger severe allergic reactions in sensitive individuals, even at concentrations below 10 ppb.
What is the Limit of Detection (LOD) and why is it important?
The Limit of Detection is 2 ppb, which represents the lowest concentration of lysozyme that can be reliably detected. This sensitivity is critical for allergen screening as allergic reactions can occur at trace levels.
What is the effective quantitation range for this kit?
The quantitation range is 25–250 ppb, allowing for accurate and precise quantification of lysozyme concentrations within this range using a calibrated standard curve.
How should this kit be stored to maintain stability?
The kit should be stored at 2–8°C (refrigerated conditions). Under these conditions, all components maintain full activity for 24 months from the date of manufacture.
Does this kit exhibit cross-reactivity with other egg proteins?
No, this kit demonstrates no cross-reactivity with other egg proteins such as ovalbumin, ovotransferrin, ovomucoid, or livetin, ensuring specific and selective detection of lysozyme only.
What is the principle of the SENSISpec ELISA Lysozyme assay?
The kit employs a sandwich ELISA format with HRP (horseradish peroxidase) enzyme linked to detection antibodies, generating a colorimetric signal with TMB substrate that is measured at 450 nm for quantitative analysis.