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Lupin ELISA Kit SENSISpec 48 Wells Allergen Test

Fiyat Sorunuz

Highly sensitive sandwich enzyme immunoassay for lupin allergen detection in food matrices with broad cross-reactivity coverage

  • Ultra-sensitive detection across multiple food matrices
  • Comprehensive coverage of all sweet lupin species due to high cross-reactivity
  • Sandwich enzyme immunoassay format for enhanced specificity
  • Rapid 60-minute total incubation protocol
  • Low detection limits: 0.1-0.7 ppm depending on matrix
  • Quantitation range 2-30 ppm suitable for regulatory compliance
  • Minimal cross-reactivity with related legumes (<0.0004%)

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The SENSISpec Lupin ELISA kit represents a state-of-the-art detection system for lupin allergens, capable of quantifying residual levels in processed foods including sausages, baked goods, potato products, ketchup, and juices. High cross-reactivity ensures comprehensive detection across all commonly used sweet lupin species. Lupin (genus Lupinus) belongs to the Fabaceae (legume) family with seed protein content of 35-45%. Specific protein fractions in alpha-, beta-, and gamma-Conglutin demonstrate allergenic properties due to cross-reactivity with soy and peanut allergens. Lupin flour adoption in food manufacturing is increasing rapidly due to nutritional advantages. The legume is particularly prevalent in allergen-free, vegetarian, and celiac-adapted diet products. Three sweet lupin species (Lupinus albus, Lupinus angustifolius, and Lupinus luteus) dominate commercial food applications. This kit utilizes sandwich enzyme immunological detection methodology for accurate quantitative analysis.

Technical Specifications

Stock Code HU0030035
Test Principle Sandwich enzyme immunological assay
Format Microtiter plate
Total Incubation Time 60 minutes (20/20/20 minute increments)
Reference Standards 0; 2.5; 15; 30 ppm
Detection Wavelength 450 nm (HRP + TMB substrate)
Limit of Detection (LOD) Sausage 0.2 ppm | Bread 0.3 ppm | Fruit juice 0.7 ppm | Ketchup 0.1 ppm | Fried potato product 0.2 ppm
Quantitation Range 2-30 ppm
Cross-Reactivity Profile Chickpea 0.0003% | Lentil 0.0004% | Unroasted soy flour 0.07% | Roasted soy flour 0.0009%
Shelf Life 13 months
Storage Conditions 2–8°C (35–46°F)

Applications

  • Food manufacturing quality assurance and allergen verification
  • Lupin residue detection in processed meat products
  • Allergen screening in bakery and cereal-based products
  • Validation of lupin-free claims in vegetarian/vegan formulations
  • Regulatory compliance testing for food labeling requirements
  • Cross-contamination monitoring in shared processing facilities
  • Detection in plant-based dairy alternative products

Frequently Asked Questions

What is the significance of lupin allergen testing?
Lupin allergens trigger cross-reactivity with peanut and soy allergenic epitopes. The increasing use of lupin flour as a sustainable protein source necessitates robust detection methods for consumer safety and regulatory compliance, particularly in EU-regulated food products.
Does this kit detect all lupin species?
Yes, the high cross-reactivity design ensures detection across all three sweet lupin species (Lupinus albus, L. angustifolius, L. luteus) commonly used in food manufacturing.
What sample types can be analyzed?
The kit is validated for diverse food matrices including meat products, baked goods, potato products, condiments, and aqueous beverages. Matrix-specific LODs are provided.
How sensitive is this assay to cross-contamination?
Cross-reactivity with related legumes (chickpea, lentil, soy) is <0.0007%, ensuring high specificity for lupin detection even in multi-legume processing environments.