The SENSISpec Milk ELISA kit represents a highly sensitive detection system for milk proteins, based on NIST 1549 reference material. The assay is capable of quantifying casein and ß-lactoglobulin residues in food matrices and has been validated for bread, crackers, sausage, orange juice, wine, soy products, and chocolate. Testing has been validated on The BoltTM automated ELISA system. Bovine milk is one of the most critical allergenic food components, particularly for children. Even minimal quantities of bovine milk can trigger allergic reactions and may cause anaphylactic shock in severe cases. Therefore, individuals with milk allergy must strictly avoid milk and milk-containing products. Hidden milk proteins in sausages, bread, ready-made meals, and beverages present critical concerns for milk-allergic individuals. In accordance with EU Directive 2003/89/EG, milk additions must be clearly labeled on product declarations.
Technical Specifications
| Stock Code |
HU0030014 |
| Test Principle |
Sandwich enzyme immunological assay |
| Format |
Microtiter plate |
| Incubation Time |
60 minutes (20/20/20 minutes) |
| Standards |
0; 0.4; 1; 4; 10 ppm milk protein |
| Detection Wavelength |
450 nm (HRP + TMB) |
| Limit of Detection (LOD) |
0.05 ppm |
| Quantitation Range |
0.4–10 ppm |
| Cross-reactivity |
None |
| Shelf Life |
13 months |
| Storage Temperature |
2–8°C |
Applications
- Food allergen screening in processed products
- Milk protein residue quantification in bakery items
- Beverage quality control and allergen verification
- Sausage and meat product allergen testing
- Regulatory compliance testing per EU Directive 2003/89/EG
- Chocolate and confectionery allergen analysis
- Automated high-throughput allergen testing workflows
Frequently Asked Questions
What is the detection principle used in this ELISA kit?
The SENSISpec Milk ELISA uses a sandwich enzyme immunoassay principle. Capture antibodies immobilize milk proteins on the microtiter plate, detection antibodies conjugated with horseradish peroxidase (HRP) bind to the complex, and TMB substrate produces a colorimetric signal measured at 450 nm.
What food matrices has this kit been validated for?
The assay has been validated for bread, crackers, sausage, orange juice, wine, soy products, and chocolate. However, performance in other food matrices should be verified by the user.
What is the clinical significance of the 0.05 ppm detection limit?
The 0.05 ppm limit of detection is sufficiently sensitive to detect milk proteins at levels relevant for allergenic risk assessment, enabling identification of hidden milk contamination in processed foods critical for patient safety.
Is this kit compatible with automated ELISA systems?
Yes, the assay has been validated on The BoltTM automated ELISA system, enabling high-throughput screening and reducing manual handling variability.
What is the turnaround time for a complete assay?
The total incubation time is 60 minutes with a protocol of 20/20/20 minutes for the three main incubation steps, allowing results within approximately 2–3 hours including plate preparation and reading.
Does this kit show cross-reactivity with other allergens?
No cross-reactivity has been documented, ensuring specificity for milk protein detection without interference from other common food allergens.
How should the kit be stored?
Store all kit components at 2–8°C (refrigerated). With proper storage, the kit maintains its performance for 13 months from the manufacturing date. Avoid freeze-thaw cycles.