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Lysozyme ELISA Kit SENSISpec 96 Well Allergen Test

Fiyat Sorunuz

Professional-grade ELISA kit for sensitive detection of lysozyme residues in food matrices with 96-well microplate format

  • Highly sensitive detection system optimized for lysozyme allergen quantification
  • Designed specifically for wine and cheese industry applications
  • 96-well microplate format enabling high-throughput analysis
  • Rapid incubation protocol: 60 minutes total (20/20/20 minute staged approach)
  • Detection limit of 2 ppb for trace-level allergen identification
  • Quantification range 25-250 ppb covering critical allergen concentration thresholds
  • Zero cross-reactivity with structurally related egg white proteins
  • Extended 24-month shelf life with standard refrigerated storage

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The SENSISpec Lysozyme ELISA Kit represents a highly sensitive detection system specifically engineered for the quantification of lysozyme residues, particularly in wine and cheese production matrices. Derived from chicken egg (Gallus gallus), lysozyme serves as a protein-based preservative widely employed in food manufacturing. While most egg white proteins demonstrate significant allergenic potential – including ovalbumin, ovotransferrin, ovomucoid, and livetin – lysozyme represents a critical allergen often undeclared in food formulations. For sensitized individuals, consumption of lysozyme at extremely low concentrations can trigger allergic reactions, escalating to anaphylactic shock in severe cases. Proper detection and quantification of lysozyme residues is essential for allergen management and regulatory compliance in food production.

Technical Specifications

Stock Code HU0030012
Test Principle SENSISpec ELISA Lysozyme
Format Microtiter Plate
Incubation Time 60 minutes (20/20/20 minutes)
Standards 0; 25, 50; 100; 250 ppb
Detection Wavelength 450 nm (HRP + TMB)
Limit of Detection (LOD) 2 ppb
Quantification Range 25 – 250 ppb
Cross-Reactivity None
Shelf Life (Months) 24
Storage Temperature 2–8°C

Applications

  • Wine and cheese allergen residue testing and regulatory verification
  • Food production quality assurance and allergen management programs
  • Regulatory compliance monitoring for undeclared lysozyme allergens
  • High-throughput allergen screening in food manufacturing facilities
  • Contract laboratory allergen analysis and product certification
  • Food safety testing and consumer protection applications

Frequently Asked Questions

Why is lysozyme detection critical in food allergen management?
Lysozyme, derived from chicken egg, is widely used as a preservative in wine and cheese production. For egg-allergic individuals, even trace amounts can trigger allergic reactions ranging from mild symptoms to severe anaphylaxis. Accurate detection is essential for proper allergen labeling and consumer safety compliance.
What is the analytical detection capability of this kit?
The SENSISpec Lysozyme ELISA kit achieves a Limit of Detection (LOD) of 2 ppb, enabling identification and quantification of lysozyme at trace levels critical for comprehensive allergen management protocols.
What is the recommended incubation protocol?
Total incubation time is 60 minutes, structured as three sequential 20-minute stages. This staged approach optimizes antibody-antigen binding kinetics while minimizing background interference and ensuring reproducible results.
Does this assay demonstrate specificity for lysozyme?
Yes, the kit shows zero cross-reactivity with structurally related egg white allergens (ovalbumin, ovotransferrin, ovomucoid, livetin), ensuring high diagnostic specificity and accuracy for lysozyme residues.
What storage conditions maintain kit performance?
Store the complete kit at 2–8°C under standard refrigeration. When stored properly, the kit maintains full analytical sensitivity and functionality for 24 months from the date of manufacture.