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Mustard ELISA Kit SENSISpec 48 Wells: Allergen Testing

Fiyat Sorunuz

High-sensitivity detection system for mustard allergen quantification in food matrices including sausages, sauces, soups, and spice blends

  • Sandwich enzyme immunoassay principle for robust quantification
  • High sensitivity with 1 ppm limit of detection
  • Cross-reactivity assessment for related Brassica species
  • Validated on automated ELISA platforms (The Bolt™ system)
  • Thermostable protein detection capability
  • Quantification range: 2-60 ppm
  • 24-month shelf life at 2-8°C storage

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The SENSISpec Mustard ELISA kit represents a highly sensitive detection system for yellow mustard quantification, specifically optimized for residue quantification in sausages, sauces, soups, cheese, and mixed herbs. Due to high cross-reactivity profile, this assay is equally suitable for brown mustard and black mustard detection. The assay has been validated on The Bolt™ automated ELISA system. Mustard belongs to the Brassicaceae family, with seed protein fractions comprising approximately 30-35% of total protein content. Key allergenic proteins include Sin a 1 and Bra j 1, which are characteristically thermostable and resistant to various food processing conditions. Yellow mustard (Sinapis alba), brown mustard (Brassica juncea), and black mustard (Brassica nigra) are widely utilized allergens in food and beverage formulations.

Technical Specifications

Stock Code HU0030040
Test Principle Sandwich enzyme immunoassay
Format 96-well microtiter plate
Incubation Time 60 minutes (20/20/20 minutes)
Standards 0; 2.6; 20; 60 ppm
Detection Wavelength 450 nm (HRP + TMB)
Limit of Detection (LOD) 1 ppm
Quantification Range 2–60 ppm
Cross-Reactivity Profile Black mustard 50%, Brown mustard 59%, Charlock mustard 48%, Coriander 0.0006%, Canola 59%
Shelf Life 24 months
Storage Conditions 2–8°C

Applications

  • Food allergen screening in processed meat products
  • Sauce and condiment allergen quantification
  • Soup base and broth contamination testing
  • Spice blend and seasoning allergen verification
  • Cheese and dairy product allergen assessment
  • Regulatory compliance testing for food labeling
  • Cross-contamination detection in food manufacturing facilities
  • Related Brassica species identification through cross-reactivity analysis

Frequently Asked Questions

What is the difference between SENSISpec Mustard ELISA and other allergen kits?
SENSISpec Mustard ELISA demonstrates high cross-reactivity with brown and black mustard varieties while maintaining specificity for yellow mustard. The kit is validated on automated systems and capable of detecting thermostable allergen proteins that survive food processing.
Why is thermostability of mustard allergens important?
Mustard proteins Sin a 1 and Bra j 1 retain allergenic potential even after heat treatment and various food processing methods. This requires detection systems capable of quantifying processed allergen residues.
What samples can be tested with this kit?
The kit is optimized for processed food matrices including sausages, sauces, soups, cheese, and herb/spice blends where mustard may be present as ingredient or contaminant.
Is this kit suitable for automated laboratory workflows?
Yes, the assay has been validated on The Bolt™ automated ELISA system for high-throughput analysis with reproducible results.
What does the 59% cross-reactivity with canola indicate?
Canola (rapeseed) is botanically related to mustard (both Brassicaceae family members). Cross-reactivity indicates potential false-positive results when both allergens are present simultaneously; confirmatory testing may be warranted.
How long can the kit be stored?
The kit maintains stability for 24 months when stored at 2–8°C. Storage outside specified temperature range may compromise assay performance.