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Cashew ELISA Kit SENSISpec 48 Wells Allergen Test

Fiyat Sorunuz

High-sensitivity sandwich immunoassay for detection of cashew protein residues in processed food matrices

  • Sandwich enzyme immunoassay (EIA) methodology
  • Limit of Detection: 0.2 ppm
  • Quantitation range: 2–60 ppm
  • Microtitre plate format with 48 wells
  • HRP-TMB detection at 450 nm
  • Incubation time: 60 minutes (20/20/20 protocol)
  • Cross-reactivity with pistachio <4%
  • 13-month shelf life at 2–8°C storage
  • ISO/EN compliant allergen quantitation

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The SENSISpec Cashew ELISA Kit represents a highly sensitive detection system specifically engineered to identify cashew residues in complex food matrices including baked goods, cereals, ice cream, and chocolate products. Cashew seeds contain approximately 18% protein content by mass. Several of these proteins—including Vicilin Ana o 1, legume allergen Ana o 2, and albumin Ana o 3—are established allergens. Many cashew allergens demonstrate thermal stability, rendering them resistant to various industrial processing conditions, which establishes cashew as a significant food allergen of concern. Even trace quantities of cashew protein can trigger allergic responses in sensitized individuals.

Technical Specifications

Stock Code HU0030028
Test Principle Sandwich enzyme immunoassay
Format Microtitre plate (48 wells)
Incubation Time 60 minutes (20/20/20 minutes)
Reference Standards 0; 2; 6; 20; 60 ppm
Detection Wavelength 450 nm (HRP + TMB)
Limit of Detection (LOD) 0.2 ppm
Quantitation Range 2–60 ppm
Cross-Reactivity Pistachio <4%
Shelf Life 13 months
Storage Conditions 2–8°C

Applications

  • Quantitative analysis of cashew allergen in cookies and baked goods
  • Cashew protein detection in breakfast cereals
  • Allergen screening in ice cream and frozen desserts
  • Trace analysis in chocolate and confectionery products
  • Food production facility allergen monitoring and validation
  • Finished product release testing for regulatory compliance
  • Supply chain allergen risk assessment

Frequently Asked Questions

What is the detection principle of the SENSISpec Cashew ELISA Kit?
The kit employs a sandwich enzyme immunoassay format utilizing HRP (horseradish peroxidase) as the enzyme and TMB as the substrate, with colorimetric detection at 450 nm wavelength.
What is the minimum detectable level of cashew protein?
The limit of detection (LOD) is 0.2 ppm, with a reliable quantitation range of 2–60 ppm for food analysis applications.
What storage conditions are required?
The kit must be stored at 2–8°C (refrigerated conditions) with a shelf life of 13 months from manufacture date.
Is the assay compatible with heat-processed foods?
Yes, the assay detects heat-stable cashew allergen epitopes, making it suitable for thermal-processed products including baked goods, roasted nuts, and chocolate formulations.
How long does the complete assay protocol require?
Total incubation time is 60 minutes, structured as three sequential 20-minute incubation steps, allowing completion within a single laboratory shift.