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SENSISpec Cashew ELISA Kit 96 Well – Allergen Testing

Fiyat Sorunuz

Highly sensitive sandwich enzyme immunoassay kit for cashew allergen detection in food products

  • Sandwich enzyme immunoassay (ELISA) method for accurate allergen detection
  • Detection limit of 0.2 ppm with quantification range of 2-60 ppm
  • Heat-stable protein detection resistant to industrial processing conditions
  • Incubation time of 60 minutes with optimized protocol (20/20/20 minutes)
  • Microtitre plate format compatible with standard ELISA readers
  • Standard reference points at 0, 2, 6, 20, and 60 ppm
  • Minimal cross-reactivity with peanuts (<4%)
  • 13-month shelf life with simple 2-8°C storage requirements

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2-3 Gün

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1-3 Gün

Ücretsiz Kargo

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The SENSISpec Cashew ELISA Kit represents a highly sensitive detection system specifically designed to identify cashew residues in cookies, cereals, ice cream, and chocolate products. Cashew seeds contain approximately 18% protein content, with several proteins including Vicilin Ana o 1, Legume Ana o 2, and Albumin Ana o 3 recognized as allergens. Many of these proteins are heat-stable, making them resistant to various manufacturing processes, which establishes cashew as a significant food allergen. Even minimal quantities of cashew can trigger allergic reactions, making reliable detection and quantification essential for food safety and labeling compliance.

Technical Specifications

Stock Code HU0030004
Test Principle Sandwich enzyme immunological assay
Format Microtitre plate
Incubation Time 60 minutes (20/20/20 minutes)
Standards 0; 2; 6; 20; 60 ppm
Detection Wavelength 450 nm (HRP + TMB)
Limit of Detection (LOD) 0.2 ppm
Quantification Range 2 – 60 ppm
Cross-Reactivity Peanut < 4%
Shelf Life 13 months
Storage Temperature 2–8°C

Applications

  • Food allergen screening in baked goods and confectionery products
  • Cashew residue detection in cereals and grain-based foods
  • Quality assurance testing in ice cream and frozen dessert manufacturing
  • Chocolate and cocoa product allergen compliance testing
  • Supply chain monitoring and ingredient verification
  • Food safety and regulatory compliance documentation
  • Allergen cross-contamination detection in shared manufacturing facilities

Frequently Asked Questions

What makes this ELISA kit suitable for cashew allergen detection?
The SENSISpec kit uses a sandwich enzyme immunoassay method specifically optimized for cashew proteins, achieving a detection limit of 0.2 ppm and quantification range of 2-60 ppm. This sensitivity is critical because even minimal cashew quantities can trigger allergic reactions.
Why are heat-stable proteins important in cashew allergen testing?
Cashew proteins such as Vicilin Ana o 1, Legume Ana o 2, and Albumin Ana o 3 are thermally stable, remaining allergenic even after high-temperature processing. This requires reliable detection methods that account for protein stability across various manufacturing conditions.
What is the significance of the 0.2 ppm detection limit?
The 0.2 ppm limit of detection enables identification of cashew residues at trace levels, supporting stringent allergen labeling requirements and protecting sensitive individuals in food products where cross-contamination may occur.
How does the 20/20/20 minute incubation protocol work?
The optimized 60-minute total incubation comprises three sequential 20-minute steps, allowing adequate time for optimal antibody-antigen binding and enzymatic reaction while maintaining assay sensitivity and specificity.
What does cross-reactivity <4% with peanuts indicate?
The minimal cross-reactivity (<4%) with peanuts demonstrates high assay specificity for cashew allergens despite both legumes sharing protein structures. This ensures accurate allergen identification without false positives from related tree nuts.
How should the kit be stored to maintain stability?
Store at 2–8°C to maintain the 13-month shelf life. Proper refrigerated storage preserves antibody activity and kit performance for reliable allergen detection throughout the designated shelf life period.