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SENSISpec ELISA Cashew Allergen 48-Well

Fiyat Sorunuz

Highly sensitive sandwich ELISA kit for detection and quantification of cashew allergens in food matrices

  • Sandwich enzyme immunoassay principle
  • Detection range: 2-60 ppm (0.2 ppm LOD)
  • Microtitre plate format with 48 wells
  • Total incubation time: 60 minutes (20/20/20 min protocol)
  • HRP/TMB detection at 450 nm
  • Heat-stable allergen detection
  • Low cross-reactivity with peanut (4%)
  • 13-month shelf life at 2-8°C

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SENSISpec Cashew ELISA represents a highly sensitive detection system for cashew allergen residues, particularly in cookie, cereal, ice cream, and chocolate matrices. With protein content accounting for approximately 18% of cashew seeds, several allergen proteins including vicilin Ana o 1, legumin Ana o 2, and albumin Ana o 3 are recognized as major allergens. These proteins exhibit heat stability, rendering them resistant to various production processes. Even trace amounts of cashew allergen can trigger allergic reactions potentially resulting in anaphylactic shock. Cross-contamination frequently occurs as a consequence of production processes, necessitating a sensitive detection system for cashew residues in food commodities.

Technical Specifications

SKU HU0030028
Test Principle Sandwich enzyme immunoassay
Format Microtitre plate
Incubation Time 60 minutes (20/20/20 min)
Standards 0, 2, 6, 20, 60 ppm
Detection Method 450 nm (HRP + TMB)
Limit of Detection (LOD) 0.2 ppm
Quantitation Range 2-60 ppm
Cross-Reactivity Peanut 4%
Shelf Life 13 months
Storage Conditions 2-8°C

Applications

  • Food allergen detection in bakery products (cookies, breads)
  • Cereal and grain product testing
  • Ice cream and dairy product analysis
  • Chocolate and confectionery allergen screening
  • Cross-contamination assessment in food production
  • Regulatory compliance testing (food labeling)
  • Supply chain verification for cashew-free claims

Frequently Asked Questions

What are the major cashew allergen proteins detected?
The kit detects vicilin (Ana o 1), legumin (Ana o 2), and albumin (Ana o 3) — the primary allergenic proteins in cashew seeds comprising approximately 18% of total seed protein.
Are heat-treated cashew allergens still detectable?
Yes. These allergen proteins exhibit significant heat stability, making them resistant to various thermal processing methods, ensuring reliable detection across different food production processes.
What is the sensitivity of this assay?
The assay provides a limit of detection (LOD) of 0.2 ppm with a quantitation range of 2-60 ppm, enabling highly sensitive detection of trace cashew allergen residues.
Does this kit cross-react with other nuts?
Cross-reactivity with peanut is minimal at 4%, providing excellent specificity for cashew allergen detection with minimal false-positive results.
How is incubation performed?
Total incubation time is 60 minutes distributed as 20/20/20 minutes across three sequential incubation steps in the sandwich immunoassay protocol.