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SENSISpec ELISA Kit Shellfish (Tropomyosin) 48-Well Allergen Test

Fiyat Sorunuz

High-sensitivity ELISA kit for Tropomyosin detection in shellfish and seafood products

  • Sandwich enzyme immunoassay principle for specific Tropomyosin detection
  • 96-well microtitre plate format with 48 test wells
  • Rapid incubation protocol: 60 minutes total (20/20/20 minutes)
  • Detection at 450 nm using HRP + TMB substrate system
  • Quantification range 20–400 ppb with no cross-reactivity
  • Low limits of detection: 0.9 ppb (baked goods) to 10.3 ppb (meat)
  • 24-month shelf life with 2–8°C storage stability

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The SENSISpec Shellfish (Tropomyosin) ELISA kit represents an exceptionally sensitive detection system for Tropomyosin (from Penaeus indicus) and is specifically designed to determine the concentration of shellfish residues in fish products, soups, sauces, baked goods, and meat products. Shellfish constitute a major food allergen group due to cross-reactivity with house dust mites. Among shellfish species, Tropomyosin is the most clinically significant protein, accounting for approximately 20% of total protein in cooked shellfish extracts. Cross-contamination during manufacturing processes is frequently detected, making quantification of shellfish residues in food products essential for allergen labeling compliance.

Technical Specifications

Stock Code HU0030030
Test Principle Sandwich enzyme immunoassay
Format Microtitre plate
Incubation Time 60 minutes (20/20/20 minutes)
Standards 0, 20, 60, 200, 400 ppb
Detection 450 nm (HRP + TMB)
Limit of Detection (LOD) Soy sauce: 1.7 ppb; Vegetable soup: 3.6 ppb; Baked goods: 0.9 ppb; Fish: 8.5 ppb; Meat: 10.3 ppb
Quantification Range 20–400 ppb
Cross-Reactivity None
Shelf Life 24 months
Storage Condition 2–8°C

Applications

  • Food allergen testing and compliance in seafood products
  • Cross-contamination detection in manufacturing facilities
  • Quality assurance screening in multi-allergen food production
  • Regulatory compliance testing (FDA, EU allergen labeling)
  • Clinical research on shellfish hypersensitivity
  • OEM testing for processed meat and baked goods
  • Supply chain verification for shellfish-free claims

Frequently Asked Questions

What is Tropomyosin and why is it the target allergen?
Tropomyosin is the primary protein allergen in shellfish, accounting for ~20% of total protein in cooked shellfish. It is clinically significant due to cross-reactivity with house dust mites and is the most relevant marker for shellfish allergen detection.
What is the sensitivity of this kit?
The limit of detection (LOD) varies by food matrix: Soy sauce 1.7 ppb, Vegetable soup 3.6 ppb, Baked goods 0.9 ppb, Fish 8.5 ppb, Meat 10.3 ppb.
Does this kit show cross-reactivity with other allergens?
No. This kit is specific for Tropomyosin and exhibits no cross-reactivity with other common allergens, ensuring accurate detection.
What is the quantification range?
The kit can reliably quantify Tropomyosin concentrations between 20–400 ppb (parts per billion).
How long is the assay protocol?
Total incubation time is 60 minutes, distributed as three 20-minute intervals, enabling same-day results.
What storage conditions are required?
Store at 2–8°C. The kit maintains stability for 24 months under these conditions.