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Macadamia ELISA Kit SENSISpec 48 Wells Allergen Test

Fiyat Sorunuz

High-sensitivity sandwich enzyme immunoassay for macadamia allergen detection in food matrices

  • Sandwich enzyme-linked immunosorbent assay (ELISA) methodology
  • Microtiter plate format with 48-well configuration
  • Detection limit of 0.1 ppm enabling trace allergen identification
  • Quantitation range 1–40 ppm with five-point calibration standard set
  • Minimal cross-reactivity: Hazelnut 0.0002%, Walnut 0.001%
  • Rapid 60-minute total incubation protocol
  • Extended shelf life of 13 months at 2–8°C storage

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SENSISpec Macadamia nut ELISA kit represents a highly sensitive detection system specifically designed for macadamia allergen quantification. The assay demonstrates reliable capability in determining residual macadamia protein concentrations in processed food matrices including cookies, cereals, ice cream, chocolate confectionery, and meat products. Macadamia nut protein content comprises approximately 7.9% by mass, with certain protein fractions exhibiting allergenic potential. While macadamia-specific allergies remain relatively uncommon, clinical manifestations can be pronounced in susceptible individuals. For patients with diagnosed macadamia nut allergy, detection of hidden allergen residues in food formulations represents a critical safety consideration.

Technical Specifications

Stock Code HU0030037
Test Principle Sandwich enzyme immunological assay
Format Microtiter plate
Incubation Time 60 minutes (20/20/20 minutes)
Standards 0; 1; 4; 10; 40 ppm
Detection Wavelength 450 nm (HRP + TMB)
Limit of Detection (LOD) 0.1 ppm
Quantitation Range 1–40 ppm
Cross-Reactivities Hazelnut 0.0002%, Walnut 0.001%
Shelf Life 13 months
Storage Temperature 2–8°C

Applications

  • Food allergen screening and compliance testing
  • Quality assurance in food manufacturing facilities
  • Cross-contamination risk assessment in shared production environments
  • Macadamia residue quantification in processed foods
  • Allergen management in food product development
  • Regulatory compliance verification (food labeling standards)

Frequently Asked Questions

What is the minimum detectable macadamia allergen concentration?
The assay limit of detection (LOD) is 0.1 ppm, enabling identification of trace macadamia protein residues in food matrices.
Are results suitable for quantitative allergen reporting?
Yes. The kit provides accurate quantitation across the 1–40 ppm range using the five-point calibration standard set (0, 1, 4, 10, 40 ppm).
What sample types are compatible with this assay?
The methodology is validated for processed food matrices including cookies, cereals, confectionery products, ice cream formulations, and processed meat products.
How does the kit perform with cross-reactive tree nuts?
Cross-reactivity is minimal: Hazelnut 0.0002% and Walnut 0.001%, providing high allergen specificity for macadamia detection.
What is the total assay time?
Total incubation requires 60 minutes, divided into three 20-minute incubation steps, plus sample preparation time.
What storage conditions ensure optimal kit stability?
Store kit components at 2–8°C. Under proper storage conditions, the kit maintains stability for 13 months from the manufacturing date.