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SENSISpec ELISA Milk (Casein) Allergen 48-Well

Fiyat Sorunuz

High-sensitivity ELISA kit for milk protein detection in food matrices based on NIST 1549 reference material

  • High sensitivity detection of milk allergens in food matrices
  • Based on NIST 1549 certified reference material
  • Validated for multiple food applications
  • Sandwich enzyme immunoassay format
  • Low detection limit of 0.05 ppm
  • Compliance with EU Directive 2003/89/EG

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Cow's milk is one of the most important allergenic food components, particularly for children. Even minute quantities of bovine milk can trigger allergic reactions that may lead to anaphylactic shock in severe cases. Consequently, individuals with milk allergy must strictly avoid milk and milk-containing food products. Hidden milk proteins in products such as cookies, ready-to-eat foods, and beverages present a critical concern for milk-allergic consumers.

Pursuant to EU Directive 2003/89/EG, bovine milk addition must be declared on product labels. Sensitive detection systems are required for identifying bovine milk in food matrices. Approximately 80% of bovine milk proteins consist of casein, while β-lactoglobulin, a major whey allergen, represents more than 10% of total protein content.

SENSISpec Milk ELISA represents a highly sensitive detection system for milk proteins based on NIST 1549 reference material. The assay quantifies casein and β-lactoglobulin residues in food samples and has been validated for cookies, bread crumbs, sausage, orange juice, wine, soy products, and chocolate matrices.

Technical Specifications

SKU HU0030038
Test Principle Sandwich enzyme immunoassay
Format Microtitre plate
Incubation Time 60 min (20/20/20 min)
Standards 0; 0.4; 1; 4; 10 ppm milk protein
Detection 450 nm (HRP + TMB)
Detection Limit (LOD) 0.05 ppm
Quantitation Range 0.4 – 10 ppm
Cross-Reactivity None
Shelf Life 13 months
Storage Temperature 2-8 °C

Applications

  • Detection of milk allergens in bakery products
  • Verification of milk residues in processed foods
  • Analysis of hidden milk proteins in beverages
  • Quality control in dairy-free product manufacturing
  • Allergen management in sausage and meat products
  • Compliance testing for EU food labeling regulations
  • Validation of milk contamination in soy-based products
  • Analysis of chocolate and confectionery matrices

Frequently Asked Questions

What is the detection principle used in this ELISA kit?
SENSISpec Milk ELISA utilizes a sandwich enzyme immunoassay principle for highly sensitive quantification of milk proteins with a detection limit of 0.05 ppm.
Is this kit suitable for detecting milk allergens in all food matrices?
The assay has been validated for cookies, bread crumbs, sausage, orange juice, wine, soy products, and chocolate. Matrix-specific validation may be required for other food applications.
What is the quantitation range of this assay?
The linear quantitation range is 0.4 – 10 ppm milk protein, with a detection limit of 0.05 ppm.
Does this kit show cross-reactivity with other allergens?
No, the kit demonstrates no cross-reactivity with other allergens, ensuring specificity for milk protein detection.
What is the shelf life and proper storage condition?
The kit has a shelf life of 13 months when stored at 2-8 °C (refrigerated conditions).