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SENSISpec ELISA Macadamia Nut Allergen 48 Wells

Fiyat Sorunuz

Highly sensitive sandwich ELISA kit for detection and quantification of macadamia nut allergen residues in food matrices

  • Detection limit (LOD) of 0.1 ppm for reliable trace analysis
  • Quantitation range of 1-40 ppm for precise residue quantification
  • Rapid incubation protocol: 60 minutes total (20/20/20 minutes)
  • Minimal cross-reactivity: Hazelnut 0.0002%, Walnut 0.001%
  • 48-well microtiter plate format for high-throughput screening
  • HRP-TMB detection system with optical readout at 450 nm

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The protein fraction in macadamia nuts is approximately 7.9%, which is relatively low compared to other tree nuts. Some of these proteins are known allergens. While macadamia nut allergies are relatively uncommon, their severity can vary significantly. Even trace amounts of macadamia nuts can trigger allergic reactions that, in severe cases, may lead to anaphylactic shock.

Cross-contamination frequently occurs as a result of production processes, which explains why the presence of macadamia nut residues in food products cannot be ruled out in many cases. Consequently, sensitive detection systems are essential for identifying macadamia nut residues in food matrices.

The SENSISpec Macadamia Nut ELISA represents a highly sensitive detection system specifically designed to quantify residue levels in cookies, cereals, ice cream, chocolate, and other food products.

Technical Specifications

SKU HU0030037
Test Principle Sandwich Enzyme Immunoassay
Format Microtiter Plate (48 wells)
Incubation Time 60 minutes (20/20/20 minutes)
Standards 0; 1; 4; 10; 40 ppm
Detection Wavelength 450 nm (HRP + TMB)
Limit of Detection (LOD) 0.1 ppm
Quantitation Range 1-40 ppm
Cross-Reactivities Hazelnut 0.0002%; Walnut 0.001%
Shelf Life 13 months
Storage Temperature 2-8°C

Applications

  • Quantification of macadamia nut allergen residues in processed foods
  • Quality control and allergen management in food manufacturing facilities
  • Detection of cross-contamination in production environments
  • Compliance testing for food safety regulations and allergen labeling requirements
  • Analysis of cookies, cereals, ice cream, and chocolate products
  • Residue monitoring in facilities processing tree nuts

Frequently Asked Questions

What is the minimum detectable concentration of macadamia nut allergen?
The limit of detection (LOD) is 0.1 ppm, enabling reliable identification of trace amounts of macadamia nut protein residues in food matrices.
What is the quantitative measurement range of this ELISA kit?
The quantitation range is 1-40 ppm, providing accurate quantification of macadamia allergen concentrations within this interval.
How long is the total assay time?
The complete incubation protocol requires 60 minutes, with optimized timing of 20 minutes for each incubation step.
Are there cross-reactions with other tree nuts?
Cross-reactivity is minimal: Hazelnut 0.0002% and Walnut 0.001%, demonstrating high specificity for macadamia allergen detection.
How should the kit be stored?
Store at 2-8°C. The kit maintains stability for 13 months under proper storage conditions.
Which food products can be analyzed with this kit?
The kit is suitable for analyzing cookies, cereals, ice cream, chocolate, and other processed foods that may contain macadamia nut residues.