Stoklu siparişleriniz aynı gün kargoda!

SENSISpec ELISA Lupin Allergen 96-Well

Fiyat Sorunuz

Highly sensitive ELISA kit for rapid detection and quantification of lupin allergens in food samples

  • Sandwich enzyme immunoassay format for reliable allergen quantification
  • Detection limit: 0.1-0.7 ppm depending on food matrix (ketchup 0.1 ppm, sausage 0.2 ppm, bread 0.3 ppm, orange juice 0.7 ppm, croquette 0.2 ppm)
  • Quantitation range: 2-30 ppm for comprehensive allergen assessment
  • High cross-reactivity with all three major food-grade lupin species (L. albus, L. angustifolius, L. luteus)
  • Minimal cross-reactivity with other legumes: Chickpea 0.0003%, Lentil 0.0004%, Soy flour (unroasted) 0.07%, Soy flour (roasted) 0.0009%
  • 96-well microtiter plate format for efficient batch testing
  • Total incubation time: 60 minutes (structured as 20/20/20 minute segments)
  • Detection at 450 nm using HRP + TMB substrate system
  • 13-month shelf life at standard laboratory storage conditions (2-8°C)

Kargolama ve Teslimat

  • Kurye Teslimatı

Kuryemiz belirtilen adrese teslimat yapacaktır.

2-3 Gün

Ü.A. Kargo

  • Aras Kargo Teslimatı

Aras Kargo kuryesi belirtilen adrese teslimat yapacaktır.

1-3 Gün

Ücretsiz Kargo

  • Garanti 2 yıl
  • 14 Günlük ücretsiz iade

The SENSISpec ELISA Lupin Allergen kit is a sandwich enzyme immunoassay designed for the sensitive detection of lupin proteins in food matrices. Lupin, a legume with protein content of approximately 35-45%, contains allergens primarily from the α-, β-, and γ-Conglutin fractions that cross-react with soy and peanut allergens. Due to its nutritional properties, lupin flour is increasingly used as a food ingredient, particularly in products targeted toward individuals with milk allergies, vegetarians, and celiac disease patients. The three main food-grade lupin species utilized in food production are Lupinus albus, Lupinus angustifolius, and Lupinus luteus. For individuals with lupin allergies, trace lupin allergens in foods pose a critical safety concern, as minimal quantities can trigger severe allergic reactions including anaphylactic shock. Cross-contamination during manufacturing frequently results in lupin residue presence in foods, making sensitive detection systems essential for food safety compliance. This kit enables reliable detection of lupin residues in sausages, baked goods, potato products, ketchup, and fruit juices with high cross-reactivity across all commonly used lupin species.

Technical Specifications

SKU HU0030011
Test Principle Sandwich enzyme immunoassay
Format Microtiter plate (96-well)
Incubation Time 60 min (20/20/20 min)
Standards 0; 2.5; 15; 30 ppm
Detection Wavelength 450 nm (HRP + TMB)
Detection Limit (LOD) Sausage 0.2 ppm, Bread 0.3 ppm, Orange juice 0.7 ppm, Ketchup 0.1 ppm, Croquette 0.2 ppm
Quantitation Range 2-30 ppm
Cross-reactivities Chickpea 0.0003%, Lentil 0.0004%, Soy flour (unroasted) 0.07%, Soy flour (roasted) 0.0009%
Shelf Life 13 months
Storage Conditions 2-8°C

Applications

  • Detection of lupin allergen residues in processed meat products (sausages, cured meats)
  • Allergen quantification in baked goods and grain-based foods
  • Quality control testing in juice and beverage production
  • Verification of lupin contamination in condiments (ketchup, sauces)
  • Cross-contamination monitoring in multi-allergen food facilities
  • Regulatory compliance testing for food labeling requirements
  • HACCP program validation for allergen control
  • Screening of plant-based protein ingredients used as meat alternatives
  • Third-party allergen verification testing

Frequently Asked Questions

What is lupin and why is it an allergen concern?
Lupin is a legume crop increasingly used as a food ingredient due to its high protein content (35-45%) and nutritional benefits. Its allergens (α-, β-, and γ-Conglutin fractions) cross-react with soy and peanut allergens, making it a significant concern for individuals with legume allergies. Even trace amounts can trigger severe allergic reactions.
What is the sensitivity of this ELISA kit?
The SENSISpec ELISA Lupin kit offers matrix-dependent detection limits ranging from 0.1-0.7 ppm. The lowest detection limit (0.1 ppm) is achieved in ketchup, while the highest (0.7 ppm) is in orange juice. This sensitivity allows detection of lupin residues below typical regulatory thresholds.
How does the test handle cross-reactivity with other legumes?
The kit demonstrates excellent specificity with minimal cross-reactivity: Chickpea 0.0003%, Lentil 0.0004%, Soy flour (unroasted) 0.07%, and Soy flour (roasted) 0.0009%. This ensures accurate lupin-specific detection even in facilities processing other legumes.
What food matrices has this kit been validated for?
The SENSISpec ELISA Lupin kit has been validated for sausages, bread products, potato-based foods, ketchup, croquettes, and fruit juices. The detection limits vary by matrix due to differences in sample composition and preparation procedures.
How should the kit be stored?
Store all kit components at 2-8°C (refrigerated conditions). Under proper storage conditions, the kit maintains full performance for 13 months from the date of manufacture.
Does this kit detect all lupin species?
Yes, due to high cross-reactivity among the three food-grade lupin species (Lupinus albus, Lupinus angustifolius, and Lupinus luteus), this kit reliably detects allergens from all commonly used commercial lupin varieties.