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Gluten R5 ELISA Kit SENSISpec INgezim – 96 Well Allergen Test Kit

Fiyat Sorunuz

ELISA kit using R5 monoclonal antibody for quantitative measurement of gluten from wheat, rye, and barley. Sensitive results in the 3-200 ppm range for food safety laboratories.

  • Specific detection of wheat, rye, and barley proteins using R5 Monoclonal Antibody
  • Low detection limit (LOD) of 3 ppm
  • Quantitative measurement range of 3-200 ppm
  • 96-well microtiter plate format, 130 minutes total incubation
  • Spectrophotometric reading at 450 nm
  • 12-month shelf life at 4°C, no cross-reactivity reported

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The SENSISpec INgezim Gluten R5 ELISA kit is designed for rapid and quantitative detection of gluten in food samples. It utilizes R5 Monoclonal Antibody specific to wheat, rye, and barley proteins, offering a low detection limit (LOD) of 3 ppm. This kit provides a practical solution for food manufacturers producing gluten-free products, quality control laboratories, and allergen risk management programs.

The assay operates in a 96-well microtiter plate format and is completed with a total incubation time of 130 minutes (60/60/10 minutes). Results are read spectrophotometrically at 450 nm wavelength. The kit provides quantitation using a five-point European gliadin standard ranging from 25 ng/mL to 1.56 ng/mL and delivers reliable measurements in the 3-200 ppm range. No cross-reactivity has been reported.

With proper storage at 4°C, the kit maintains a shelf life of 12 months. Results are obtained quantitatively, providing convenience in confirming regulatory compliance such as gluten-free labeling requirements (20 ppm threshold).

Technical Specifications

Manufacturer Gold Standard Diagnostics
Allergen Type Gluten
Test Principle Sandwich enzyme immunoassay (ELISA)
Format 96-well microtiter plate
Incubation Time 130 minutes (60/60/10 minutes)
Standards 25; 12.5; 6.25; 3.12; 1.56 ng/mL (European Gliadin)
Detection Wavelength 450 nm
Limit of Detection (LOD) 3 ppm gluten
Quantitative Range 3-200 ppm
Cross-Reactivity None
Shelf Life 12 months
Storage 4°C
Result Type Quantitative

Applications

  • Gluten-free product labeling compliance verification in food manufacturing
  • Routine gluten screening in food quality control laboratories
  • Raw material and finished product testing in allergen risk management programs

Frequently Asked Questions

Which cereal proteins does this kit detect?
The R5 Monoclonal Antibody is specifically designed to detect gluten proteins originating from wheat, rye, and barley.
How long does it take to obtain test results?
Total incubation time is 130 minutes (60/60/10 minutes), followed by reading at 450 nm wavelength.
What is the detection limit of the kit?
The detection limit is 3 ppm gluten with a quantitative measurement range of 3-200 ppm.