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Brazil Nut ELISA Kit SENSISpec 48 Wells Allergen Test

Fiyat Sorunuz

High-sensitivity sandwich ELISA kit for quantitative determination of Brazil nut residues in food matrices with 0.2 ppm LOD

  • Sandwich enzyme immunoassay format with high specificity
  • Limit of Detection (LOD): 0.2 ppm
  • Quantitative range: 1-40 ppm
  • No cross-reactivity with other tree nuts
  • 48-well microtiter plate format
  • Total incubation time: 60 minutes (20/20/20 min cycles)
  • 13-month shelf life at 2–8°C storage
  • HRP-TMB detection at 450 nm

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The SENSISpec Brazil Nut ELISA Kit represents a highly sensitive detection system specifically designed for the quantification of Brazil nut residues in food products such as biscuits, cereals, ice cream, chocolate, and meat products. Brazil nuts contain approximately 14% protein content, including allergenic proteins such as 2S albumin (Ber e 1) and 11S legumin (Ber e 2). Although Brazil nut allergies are relatively rare, they can be severe and may trigger anaphylactic shock even at very low concentrations. Cross-contamination during manufacturing processes is frequently observed, making residue detection in food products essential for allergen management and regulatory compliance.

Technical Specifications

SKU HU0030042
Test Principle Sandwich enzyme immunoassay
Format Microtiter plate
Incubation Time 60 minutes (20/20/20 minutes)
Standards 0; 1; 4; 10; 40 ppm
Detection Wavelength 450 nm (HRP + TMB)
Limit of Detection (LOD) 0.2 ppm
Quantitative Range 1–40 ppm
Cross-reactivity None
Shelf Life 13 months
Storage Temperature 2–8°C

Applications

  • Food allergen control in bakery and confectionery products
  • Cereal and grain-based product testing
  • Ice cream and frozen dessert allergen screening
  • Chocolate and cocoa product compliance testing
  • Processed meat and sausage allergen detection
  • Cross-contamination monitoring in multi-product facilities
  • Regulatory compliance testing for food labeling
  • Supply chain allergen verification

Frequently Asked Questions

What is the Limit of Detection (LOD) for this kit?
The LOD is 0.2 ppm, enabling detection of Brazil nut residues at very low concentrations relevant for allergen management.
What is the quantitative measurement range?
The quantitative range is 1–40 ppm, allowing accurate quantification within this concentration interval.
How long is the total testing procedure?
The total incubation time is 60 minutes, consisting of three sequential 20-minute incubation steps, allowing for efficient sample processing.
Does this kit cross-react with other tree nuts?
No, the kit exhibits no cross-reactivity with other allergens, ensuring Brazil nut-specific detection.
What storage conditions are required?
The kit must be stored at 2–8°C (refrigerated conditions) and has a shelf life of 13 months.
Which allergen proteins does this kit target?
The kit targets allergenic proteins in Brazil nuts, including 2S albumin (Ber e 1) and 11S legumin (Ber e 2).
What detection method is used?
The kit utilizes HRP-TMB (horseradish peroxidase-tetramethylbenzidine) detection, measured at 450 nm wavelength.