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SENSI Spec Crustaceans (Tropomyosin) ELISA Test Kit

Fiyat Sorunuz

High-sensitivity sandwich enzyme immunoassay for quantitative detection of crustacean allergen (tropomyosin) in food matrices

  • Highly sensitive sandwich ELISA format targeting tropomyosin allergen
  • Microtiter plate-based system with rapid 60-minute incubation protocol
  • Multiple validated food matrix LODs: soy sauce 1.7 ppb, vegetable soup 3.6 ppb, bakery products 0.9 ppb, fish 8.5 ppb, meat 10.3 ppb
  • Quantitation range 20-400 ppb covering regulatory and manufacturing thresholds
  • No cross-reactivity with non-target allergens
  • Extended 24-month shelf life for kit stability

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Ücretsiz Kargo

  • Garanti 2 yıl
  • 14 Günlük ücretsiz iade

Crustaceans represent a major food allergen group of significant regulatory concern. Tropomyosin, the principal allergenic protein found across all common crustacean species, comprises approximately 20% of total protein content in cooked crustacean extracts. Cross-contamination during food processing is a widespread occurrence, making it difficult to exclude crustacean residues in many food products. Consequently, sensitive and reliable detection systems are essential for crustacean residue quantification in food materials. SENSI Spec Crustaceans (Tropomyosin) ELISA provides highly sensitive detection of tropomyosin derived from Penaeus indicus and is specifically designed for quantification of crustacean residues in fish products, soups, sauces, bakery products, and meat preparations.

Technical Specifications

SKU HU0030030
Test Principle Sandwich enzyme immunoassay
Format Microtiter plate
Incubation Time 60 min (20/20/20 min)
Standards 0; 20, 60; 200; 400 ppb
Detection Wavelength 450 nm (HRP + TMB)
Limit of Detection (LOD) Soy sauce 1.7 ppb; Vegetable soup 3.6 ppb; Bakery products 0.9 ppb; Fish 8.5 ppb; Meat 10.3 ppb
Quantitation Range 20-400 ppb
Cross-Reactivity No
Shelf Life 24 months

Applications

  • Quantification of crustacean allergen in fish products and seafood processing
  • Detection of tropomyosin residues in soups and broth-based products
  • Allergen verification in sauces and condiments
  • Cross-contamination monitoring in bakery and meat product facilities
  • Regulatory compliance testing for crustacean declaration labeling

Frequently Asked Questions

Why is tropomyosin detection critical in food allergen analysis?
Tropomyosin is the primary allergenic protein in crustaceans, constituting ~20% of total protein in cooked extracts. It is highly conserved across crustacean species and is a major IgE-binding allergen responsible for hypersensitivity reactions in susceptible individuals.
What food matrices can be tested with this ELISA kit?
The kit is validated for fish products, soups, sauces, bakery products, and meat preparations. Each matrix has distinct LOD values optimized through method validation studies.
What does the quantitation range 20-400 ppb represent?
The 20-400 ppb range represents the linear dynamic range of the assay where quantitative results with acceptable accuracy and precision can be reliably obtained. Results outside this range may require sample dilution.
Is HRP+TMB detection suitable for standard laboratory readers?
Yes, HRP (horseradish peroxidase) detection at 450 nm with TMB substrate is compatible with all standard ELISA microplate readers, making the assay accessible to routine laboratory environments.
What is the significance of the 24-month shelf life?
The 24-month shelf life ensures long-term reagent stability when stored under recommended conditions, reducing frequent kit replenishment and optimizing laboratory inventory management.