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SENSISpec ELISA Milk (Casein) Allergen 96-Well Kit

Fiyat Sorunuz

Highly sensitive sandwich enzyme immunoassay for milk protein detection in food matrices, based on NIST 1549 reference material

  • Sandwich enzyme immunoassay format for reliable milk protein quantification
  • Validated on NIST 1549 reference material
  • Low detection limit of 0.05 ppm ensures sensitivity for allergenic residues
  • Quantitation range 0.4–10 ppm covers typical analytical requirements
  • Multi-matrix validation including cookies, bread, processed meats, and beverages
  • No cross-reactivity with other allergens
  • 13-month shelf life with 2–8°C storage stability

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Bovine milk is one of the most important allergenic food components, particularly for children. Even trace amounts of milk protein can trigger severe allergic reactions, up to anaphylactic shock. Individuals with milk allergy must strictly avoid milk and milk-containing food products. Hidden milk proteins in cookies, ready-to-eat products, and beverages represent a critical concern for allergic consumers. According to EU Directive 2003/89/EC, bovine milk additives must be labeled. Sensitive detection systems are required for the identification of milk proteins in food matrices. Approximately 80% of bovine milk proteins consist of casein, while β-lactoglobulin, the main whey allergen, represents more than 10% of total protein content. The SENSISpec Milk ELISA represents a highly sensitive detection system for milk proteins based on NIST 1549 reference material. The test can simultaneously quantify casein and β-lactoglobulin residues in food samples and has been validated for cookies, bread crumbs, sausage, orange juice, wine, soy products, and chocolate.

Technical Specifications

SKU HU0030014
Test Principle Sandwich enzyme immunoassay
Format Microtiter plate
Incubation Time 60 min (20/20/20 min)
Standards 0; 0.4; 1; 4; 10 ppm milk protein
Detection Wavelength 450 nm (HRP + TMB)
Limit of Detection (LOD) 0.05 ppm
Quantitation Range 0.4–10 ppm
Cross-Reactivity No
Shelf Life 13 months
Storage Temperature 2–8°C

Applications

  • Detection and quantification of milk proteins in processed foods
  • Validation of casein content in bakery products
  • Analysis of dairy residues in meat-based products
  • Quality control in beverage manufacturing (juice, wine)
  • Allergen screening in chocolate and confectionery
  • Compliance testing for EU Directive 2003/89/EC labeling requirements
  • Quantification of milk allergen in plant-based alternatives
  • Cross-contamination assessment in dedicated allergen-free facilities

Frequently Asked Questions

What is the principle of the SENSISpec Milk ELISA test?
The test employs a sandwich enzyme immunoassay principle, utilizing capture and detection antibodies specific to milk proteins. Horseradish peroxidase (HRP) and 3,3',5,5'-tetramethylbenzidine (TMB) substrate enable colorimetric detection at 450 nm wavelength.
What is the detection limit of this assay?
The limit of detection (LOD) is 0.05 ppm for milk protein, making it suitable for detection of allergenic residues even in traces.
In which food matrices has this test been validated?
Validation has been completed in cookies, bread crumbs, sausage, orange juice, wine, soy products, and chocolate matrices.
Can this test differentiate between casein and β-lactoglobulin?
Yes, the assay can quantify both casein and β-lactoglobulin residues simultaneously, as both represent major milk allergens.
What storage conditions are required?
The kit should be stored at 2–8°C. Under these conditions, shelf life is 13 months from the date of manufacture.
Does the test show cross-reactivity with other allergens?
No, cross-reactivity with other common allergens has not been observed, ensuring specificity for bovine milk proteins.