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Beta-Lactoglobulin ELISA Kit SENSISpec 48 Wells – Allergen Testing

Fiyat Sorunuz

SENSISpec ELISA Beta-Lactoglobulin 48-Well Kit: Sensitive Detection of Bovine Milk Residues

  • Highly sensitive sandwich enzyme immunoassay for bovine milk detection
  • Validated 48-well microtiter plate format
  • Suitable for multiple food matrices: bakery products, processed meats, beverages, and chocolate
  • Detection limit: 1.5 ppb with quantitative range 10-400 ppb
  • Excellent specificity with minimal cross-reactivity to ovine and caprine milk
  • Rapid incubation protocol: 60 minutes total (20/20/20 minutes)
  • Long shelf life of 13 months at 2-8°C

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The SENSISpec ß-Lactoglobulin ELISA kit represents a highly sensitive detection system, particularly effective for identification and quantification of bovine milk residues in cookies, cereals, sausages, orange juice, wine, soy products, and chocolate. This assay has been validated on the BoltTM automatic ELISA system. Bovine milk is one of the most important allergenic food ingredients, especially for children. Even at very low concentrations, bovine milk can trigger allergic reactions and, in severe cases, may lead to anaphylactic shock. Hidden milk proteins in sausages, cookies, ready-to-eat foods, or beverages present a critical concern for milk-allergic individuals. Approximately 20% of bovine milk proteins are whey proteins, with the major whey protein fraction being beta-lactoglobulin.

Technical Specifications

SKU HU0030026
Test Principle Sandwich enzyme immunoassay
Format Microtiter plate
Incubation Time 60 minutes (20/20/20 minutes)
Standards 0; 10, 40; 100; 400 ppb
Detection Wavelength 450 nm (HRP + TMB)
Limit of Detection (LOD) 1.5 ppb
Quantitative Range 10 – 400 ppb
Cross-reactivity: Sheep Milk < 0.2%
Cross-reactivity: Goat Milk < 0.002%
Cross-reactivity: Casein < 0.02%
Shelf Life 13 months
Storage Conditions 2–8°C

Applications

  • Detection of bovine milk residues in processed foods
  • Allergen screening in bakery and confectionery products
  • Quality control testing in food manufacturing
  • Milk contamination assessment in sausage and meat products
  • Beverage allergen compliance testing
  • Soy product milk cross-contamination detection
  • Regulatory compliance and labeling verification

Frequently Asked Questions

What is the primary target allergen detected by this kit?
The kit specifically detects beta-lactoglobulin (ß-Lg), a major whey protein component of bovine milk, which accounts for approximately 20% of bovine milk proteins.
Which food matrices have been validated for this assay?
This ELISA kit has been validated for detection of bovine milk residues in cookies, cereals, sausages, orange juice, wine, soy products, and chocolate.
What is the limit of detection (LOD)?
The limit of detection is 1.5 ppb (parts per billion) with a quantitative working range of 10-400 ppb.
How much cross-reactivity does this kit show with other milk sources?
Cross-reactivity is minimal: sheep milk < 0.2%, goat milk < 0.002%, and casein < 0.02%, demonstrating excellent specificity for bovine milk.
What detection system is required?
The kit uses HRP (horseradish peroxidase) enzyme linked to TMB (tetramethylbenzidine) substrate, with absorbance measurement at 450 nm on a standard ELISA reader. Validation has been performed on the BoltTM automatic ELISA system.