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Brazil Nut ELISA Kit SENSISpec 96-Well Allergen Testing

Fiyat Sorunuz

Sensitive detection system for Brazil nut residue quantification in food matrices using sandwich enzyme immunoassay technology

  • Sandwich enzyme immunoassay (EIA) format for high specificity
  • Limit of Detection (LOD): 0.2 ppm for ultra-sensitive residue detection
  • Quantitative range: 1 - 40 ppm for comprehensive allergen profiling
  • Zero cross-reactivity with related tree nuts and legumes
  • Microtiter plate format compatible with standard ELISA readers
  • Optimized 60-minute incubation protocol (20/20/20 minutes)
  • Suitable for biscuits, cereals, ice cream, chocolate, and processed meat products
  • 13-month shelf life when stored at 2–8°C

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Ücretsiz Kargo

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  • 14 Günlük ücretsiz iade

The SENSISpec Brazil Nut ELISA kit represents a highly sensitive detection system specifically designed for quantification of Brazil nut residues in processed foods including biscuits, cereals, ice cream, chocolate, and sausages. Brazil nuts contain approximately 14% protein content, with certain proteins such as 2S albumin (Ber e 1) and 11S legumin (Ber e 2) possessing allergenic properties. While Brazil nut allergies remain relatively uncommon compared to other tree nuts, they can manifest with particular severity. Even trace amounts of Brazil nut protein can trigger allergic reactions, with severe cases potentially resulting in anaphylactic shock. Cross-contamination during manufacturing processes is frequently encountered, necessitating reliable detection methods for Brazil nut residues in food products intended for sensitive populations.

Technical Specifications

SKU HU0030018
Test Principle Sandwich enzyme immunoassay
Format Microtiter plate
Incubation Time 60 minutes (20/20/20 minutes)
Standards 0; 1; 4; 10; 40 ppm
Detection Wavelength 450 nm (HRP + TMB substrate)
Limit of Detection (LOD) 0.2 ppm
Quantitative Range 1 – 40 ppm
Cross-Reactivity None
Shelf Life 13 months
Storage Conditions 2–8°C

Applications

  • Food allergen screening in biscuit manufacturing
  • Brazil nut residue quantification in cereal products
  • Cross-contamination detection in ice cream processing
  • Allergen monitoring in chocolate and confectionery production
  • Sausage and processed meat allergen verification
  • Regulatory compliance testing for food labeling
  • Supplier material testing and validation
  • Facility sanitation verification and hygiene monitoring

Frequently Asked Questions

What is the significance of the 2S albumin (Ber e 1) and 11S legumin (Ber e 2) proteins?
These are the primary allergenic proteins found in Brazil nuts. Ber e 1 (2S albumin) and Ber e 2 (11S legumin) are responsible for triggering allergic reactions in sensitized individuals. Accurate detection of these proteins is essential for food safety and proper allergen labeling.
Why is cross-contamination monitoring important for Brazil nut residues?
Cross-contamination frequently occurs during manufacturing processes when equipment or facilities are shared with Brazil nut-containing products. Even trace amounts (below 1 ppm) can trigger severe reactions in highly sensitive individuals, including anaphylaxis. Regular monitoring ensures product safety.
What does the 0.2 ppm Limit of Detection mean?
The LOD of 0.2 ppm indicates that the kit can reliably detect Brazil nut protein concentrations as low as 0.2 parts per million, providing exceptional sensitivity for trace allergen detection in finished products and ingredient verification.
How should the kit be stored and what is its shelf life?
Store the SENSISpec Brazil Nut ELISA kit at 2–8°C (refrigerated conditions). Under proper storage, the kit maintains its analytical performance for 13 months from the date of manufacture. Do not freeze components unless specified in the package insert.
What is the advantage of the sandwich enzyme immunoassay format?
The sandwich EIA format provides high specificity and sensitivity by utilizing capture and detection antibodies specific to Brazil nut allergens. This configuration minimizes non-specific binding and reduces false positive results compared to alternative assay formats.