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Casein ELISA Kit SENSISpec 96 Wells Allergen Test

Fiyat Sorunuz

Highly sensitive detection and quantification of bovine casein residues in food and beverage matrices using sandwich enzyme immunoassay technology

  • Sandwich enzyme immunoassay principle for superior specificity and sensitivity
  • Detection limit of 0.04 ppm enables identification of trace casein contamination
  • Quantitative range 0.2–6 ppm covers regulatory and industrial testing requirements
  • Minimal cross-reactivity with ovine (<1.2%) and caprine (<1.1%) milk proteins
  • 96-well microtiter plate format enables high-throughput allergen screening
  • Incubation protocol optimized for rapid turnaround (60 minutes total)
  • 13-month shelf-life with standard 2–8°C refrigerated storage

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Bovine milk, particularly casein proteins, represents one of the most significant allergenic food components, especially for children. Even trace amounts of bovine milk casein can trigger allergic reactions and, in severe cases, anaphylactic shock. The presence of hidden milk proteins in products such as sausages, cookies, ready-to-eat foods, and beverages creates critical risk for milk-allergic individuals.

Approximately 80% of bovine milk proteins are composed of α-, β-, and κ-caseins—heat-stable allergens that constitute the primary proteinaceous fraction of bovine milk. SENSISpec Casein ELISA Kit represents a highly sensitive detection system specifically engineered for identification and quantification of bovine casein residues in cookies, bread, sausages, orange juice, wine, soy-based products, and chocolate formulations.

Technical Specifications

SKU HU0030003
Test Principle Sandwich enzyme immunoassay
Format Microtiter plate
Incubation Time 60 minutes (20/20/20 minutes)
Reference Standards 0; 0.2; 0.6; 2; 6 ppm
Detection Wavelength 450 nm (HRP + TMB)
Limit of Detection (LOD) 0.04 ppm
Quantitative Range 0.2–6 ppm
Cross-Reactivity Ovine milk <1.2%, Caprine milk <1.1%
Shelf Life 13 months
Storage Temperature 2–8°C

Applications

  • Allergen screening in bakery and confectionery products
  • Milk protein contamination analysis in meat processing facilities
  • Quality control testing for beverage manufacturers (juices, wines)
  • Soy-based food product verification for casein cross-contamination
  • Food safety compliance testing for regulated food production
  • Research laboratories conducting allergenicity assessments
  • Third-party certification and audit laboratory testing

Frequently Asked Questions

What is the clinical significance of bovine casein allergen detection?
Bovine casein represents the most prevalent heat-stable milk allergen. Detection capability is essential for protecting individuals with IgE-mediated milk allergy from potentially life-threatening exposure through hidden food sources.
Why is the 0.04 ppm limit of detection important?
The LOD of 0.04 ppm ensures sensitivity sufficient to identify casein residues below typical regulatory thresholds, enabling manufacturers to verify allergen management effectiveness in cleaning validation and product safety protocols.
How does the kit minimize false positives from other milk sources?
Cross-reactivity specifications (<1.2% with ovine milk, <1.1% with caprine milk) demonstrate that the assay is specifically engineered to detect bovine casein while maintaining minimal interference from other mammalian milk proteins.
Is the 13-month shelf life adequate for routine laboratory use?
Yes; the extended shelf-life supports consistent inventory management and cost-effectiveness for laboratories performing regular allergen screening programs with standard 2–8°C refrigeration.