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Casein ELISA Kit SENSISpec 48 Wells Allergen Test

Fiyat Sorunuz

Sensitive detection system for bovine casein residues in food matrices using sandwich enzyme immunoassay

  • Sandwich enzyme immunoassay format with high sensitivity
  • Detection limit of 0.04 ppm for trace casein residues
  • Suitable for diverse food matrices including processed foods and beverages
  • Heat-stable allergen detection capability
  • Minimal cross-reactivity with ovine and caprine milk proteins

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The SENSISpec Casein ELISA kit represents a highly sensitive detection system specifically designed for identification and quantification of bovine casein residues in various food products. Bovine milk proteins, particularly in children, represent one of the most significant allergenic food components. Even minute quantities of bovine milk can trigger allergic reactions and, in severe cases, anaphylactic shock. The presence of hidden milk proteins in products such as sausages, biscuits, processed foods, and beverages creates a critical concern for individuals with milk allergy. Approximately 80% of bovine milk proteins consist of caseins (α-, β-, and κ-caseins), which are heat-stable allergens representing the major fraction of bovine milk proteins. The SENSISpec Casein ELISA kit demonstrates exceptional sensitivity and specificity for detection and quantification of bovine casein residues in biscuits, bread crumbs, sausages, orange juice, wine, soy products, and chocolate.

Technical Specifications

SKU HU0030027
Test Principle Sandwich enzyme immunoassay
Format Microtiter plate
Incubation Time 60 minutes (20/20/20 minutes)
Standards 0; 0.2; 0.6; 2; 6 ppm
Detection Wavelength 450 nm (HRP + TMB)
Limit of Detection (LOD) 0.04 ppm
Quantitative Range 0.2 – 6 ppm
Cross-Reactivity Sheep milk < 1.2%, Goat milk < 1.1%
Shelf Life 13 months
Storage Conditions 2–8°C

Applications

  • Detection of casein residues in biscuits and cookies
  • Bread crumb and bakery product allergen screening
  • Processed meat and sausage casein quantification
  • Beverage analysis (orange juice, wine, soft drinks)
  • Soy product cross-contamination assessment
  • Chocolate and confectionery allergen testing
  • Quality control in milk-free food manufacturing
  • Regulatory compliance testing for allergen labeling

Frequently Asked Questions

What is the minimum detectable level of casein in this kit?
The limit of detection (LOD) is 0.04 ppm, allowing identification of trace casein residues even in low-contamination scenarios.
Can this kit detect casein from non-bovine sources?
The kit is specifically designed for bovine casein. Cross-reactivity with ovine (sheep) milk is < 1.2% and with caprine (goat) milk is < 1.1%, making it highly specific for bovine proteins.
What sample types are compatible with this ELISA kit?
The kit is validated for diverse food matrices including biscuits, bread products, processed meats, fruit juices, wines, soy products, and chocolate.
How long is the total assay time?
The total incubation time is 60 minutes, structured as three sequential 20-minute incubation steps.
What storage conditions are required?
All kit components must be stored at 2–8°C. Under these conditions, the kit maintains full activity for 13 months.
Is this kit suitable for regulatory compliance testing?
Yes, the kit's quantitative range of 0.2-6 ppm and low LOD make it suitable for food safety regulations and allergen labeling compliance in multiple jurisdictions.