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Egg White ELISA Kit SENSISpec 48-Well Allergen Test

Fiyat Sorunuz

High-sensitivity sandwich enzyme immunoassay for quantitative detection of egg white protein (ovalbumin) residues in food samples, specifically designed to identify heat-stable allergens in processed foods.

  • Sandwich enzyme immunoassay (EIA) format
  • 96-well microplate configuration
  • Detection limit (LOD): 0.05 ppm
  • Quantitation range: 0.4–10 ppm
  • Heat-resistant ovalbumin target detection
  • Application validated on automated ELISA systems (Bolt™)
  • Five-point calibration (0; 0.4; 1; 4; 10 ppm)
  • Minimal cross-reactivity (Chicken < 0.001%)
  • 24-month shelf life at 2–8°C storage

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The SENSISpec Egg White ELISA Kit represents a highly sensitive detection system for egg white protein, based on ovalbumin measurement, with demonstrated capacity to quantify egg white protein residues in foods such as pasta, baked goods, sausages, and chocolate. This assay has been validated on the Bolt™ automated ELISA system. Please note that thermal processing may result in reduced recovery rates. Chicken egg (Gallus gallus) is a protein-rich food and represents an important dietary source for humans. While egg yolk proteins possess only mild allergenic properties, numerous egg white proteins are recognized as allergens. Ovalbumin, ovotransferrin, lysozyme, and livetin, along with ovalbumin, represent the most significant allergens. Unlike other allergens, ovalbumin is heat-stable and can resist common production processes such as baking. This kit employs a sandwich enzyme immunological test principle on a 96-well microplate format with 60-minute total incubation (20/20/20 minutes) for optimal sensitivity and specificity.

Technical Specifications

Stock Code HU0030031
Test Principle Sandwich enzyme immunoassay (EIA)
Format Microtiter plate (96-well)
Total Incubation Time 60 minutes (20/20/20 minutes)
Calibration Standards 0; 0.4; 1; 4; 10 ppm
Detection Wavelength 450 nm (HRP + TMB substrate)
Limit of Detection (LOD) 0.05 ppm
Quantitation Range 0.4–10 ppm
Cross-Reactivity Chicken < 0.001%
Shelf Life 24 months
Storage Conditions 2–8°C

Applications

  • Food allergen screening in processed food products (pasta, baked goods, sausages, confectionery)
  • Quality control and regulatory compliance testing
  • Detection of egg white protein residues in cross-contamination risk assessment
  • Food safety and HACCP program implementation
  • Manufacturer allergen labeling verification
  • Research applications in food protein analysis

Frequently Asked Questions

What allergen does this kit specifically target?
This kit targets ovalbumin and other egg white proteins (ovotransferrin, lysozyme, livetin). Ovalbumin is prioritized as it is heat-stable and resistant to common food processing methods like baking.
Why focus on egg white rather than egg yolk?
Egg white proteins are known to possess significantly greater allergenic potential compared to egg yolk proteins, which have only mild allergenic properties. This makes egg white allergen detection more relevant for food safety applications.
How does thermal processing affect test results?
Heat treatment may result in reduced recovery rates. However, the ovalbumin target is more heat-stable than other egg allergens, making this kit more suitable for detection in thermally processed foods.
Is this kit suitable for automated systems?
Yes, this assay has been validated on the Bolt™ automated ELISA system and is compatible with standard automated EIA protocols.
What is the detection principle?
The kit uses a sandwich enzyme immunoassay (EIA) principle with HRP (horseradish peroxidase) enzyme detection and TMB substrate, measured at 450 nm.
What is the clinical/practical significance of the 0.05 ppm LOD?
The 0.05 ppm limit of detection allows for precise quantification of trace egg allergen residues, supporting compliance with stringent labeling regulations and allergen management protocols.