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Sesame ELISA Kit SENSISpec 48 Wells – Allergen Testing

Fiyat Sorunuz

High-sensitivity sandwich enzyme immunoassay for quantitative sesame allergen detection in food matrices. 48-well format, 2-30 ppm quantitation range.

  • Sandwich enzyme immunological assay principle with high specificity
  • 48-well microtiter plate format for efficient batch testing
  • Quantitation range: 2-30 ppm with established standards (0, 2, 5, 15, 30 ppm)
  • Rapid incubation protocol: 60 minutes total (20/20/20 minute intervals)
  • HRP-TMB detection at 450 nm wavelength
  • 18-month shelf life with stable 2–8°C storage requirements
  • Validated cross-reactivity data across multiple food matrices

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The SENSISpec Sesame ELISA kit represents a highly sensitive detection system for sesame residues and is particularly well-suited for quantification in flour products, soups, sausages, sauces, and ice cream. Sesame seeds contain a protein fraction of approximately 16-32%, with several of these proteins possessing allergenic properties, including albumins Ses i 1 and Ses i 2, and globulin Ses i 3. Due to widespread culinary applications of sesame, it is used in numerous food preparations. For individuals with sesame allergy, hidden sesame allergens in food products represent a critical concern. Even minute quantities of sesame can trigger allergic reactions that, in severe cases, may lead to anaphylactic shock. Therefore, individuals with sesame allergy must strictly avoid consumption of sesame-containing foods. Cross-contamination, typically a consequence of production processes, presents an additional risk requiring careful control and monitoring.

Technical Specifications

SKU HU0030046
Test Principle Sandwich enzyme immunoassay
Format 48-well Microtiter plate
Incubation Time 60 minutes (20/20/20 minutes)
Standards 0; 2; 5; 15; 30 ppm
Detection Wavelength 450 nm (HRP + TMB)
Quantitation Range 2–30 ppm
Cross-Reactivity Data Soup 0.2 ppm, Ice cream 0.2 ppm, Sausage 0.2 ppm, Salad dressing 0.2 ppm, Cracker 0.2 ppm, Oat 0.0003%, Bean 0.0003%
Shelf Life 18 months
Storage Conditions 2–8°C

Applications

  • Food allergen screening and quantification in processed foods
  • Sesame residue detection in flour-based products and bakery items
  • Allergen monitoring in soup and sauce manufacturing
  • Quality control testing in ice cream and frozen dessert production
  • Cross-contamination assessment in multi-allergen food facilities
  • Regulatory compliance testing for food labeling requirements
  • Research and development of sesame-free formulations

Frequently Asked Questions

What is the principle behind this ELISA assay?
The kit employs a sandwich enzyme immunoassay principle, where capture antibodies immobilize sesame antigens, followed by detection antibodies conjugated with horseradish peroxidase (HRP). TMB substrate generates a measurable colorimetric signal at 450 nm.
Which sesame proteins does this kit detect?
The assay targets major sesame allergen proteins, including albumins Ses i 1 and Ses i 2, and globulin Ses i 3, which constitute a significant portion of the 16-32% protein fraction present in sesame seeds.
What food matrices have been validated?
Cross-reactivity has been evaluated in soups, ice cream, sausages, salad dressings, crackers, oats, and beans, with documented cross-reactivity values for method development and validation.
What is the minimum detectable sesame level?
The lower limit of the quantitation range is 2 ppm, with reliable quantification extending to 30 ppm. Detection sensitivity is optimized for common food application scenarios.
What are the storage requirements?
Store the kit at 2–8°C (refrigerated conditions). Under proper storage, the kit maintains stability for 18 months from the date of manufacture.
How long does the complete assay take?
Total incubation time is 60 minutes, consisting of three 20-minute incubation intervals, allowing for efficient processing of multiple samples in a single working session.