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TBE 10x Electrophoresis Buffer (Clear)

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10x concentrated TBE buffer for nucleic acid separation in agarose and polyacrylamide gel electrophoresis. Dilute to 1x working concentration before use.

  • 10x concentrate, dilute to 1x before use
  • 1x composition: 90 mM Tris, 90 mM boric acid, 2 mM EDTA
  • Filter sterilized
  • Prepared in ultrapure water
  • Compatible with agarose and polyacrylamide gel electrophoresis
  • Fresh 1x buffer preparation recommended for each run

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TBE 10x Electrophoresis Buffer is a concentrated buffer solution formulated for laboratories performing DNA/RNA separation in agarose and polyacrylamide gels. Prepared with ultrapure water and filter sterilized, it is ready for direct dilution and application.

The product is a standard Tris-Borate-EDTA formulation that must be diluted to 1x working concentration prior to electrophoresis. For optimal band quality and reproducibility, preparation of fresh 1x buffer for each run is recommended. This buffering system provides a practical solution for laboratories conducting genetic analysis, PCR product verification, and nucleic acid purity studies.

Technical Specifications

Concentration 10x
1x Composition 90 mM Tris, 90 mM boric acid, 2 mM EDTA
Sterilization Filter sterilized
Solvent Ultrapure water
SKU TBE500, TBE1000

Applications

  • Agarose gel electrophoresis
  • Polyacrylamide gel electrophoresis
  • DNA/RNA fragment separation
  • PCR product verification

Frequently Asked Questions

How is TBE 10x buffer used?
Dilute with ultrapure water to 1x working concentration before each electrophoresis run.
Is fresh buffer required for each run?
Yes, preparation of fresh 1x buffer for each electrophoresis application is recommended to achieve optimal results.
What is the composition of 1x buffer?
The 1x buffer contains 90 mM Tris, 90 mM boric acid, and 2 mM EDTA.