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ClearBand 6x DNA Loading Dye Green

Fiyat Sorunuz

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Pre-mixed loading buffer with dual tracking dyes for DNA visualization in gel electrophoresis applications.

Dual tracking dyes (Xylene Cyanol FF and Orange G) for precise DNA migration visualization
Orange G tracks at ~50 bp in agarose gels for improved sample monitoring
Minimizes background interference compared to traditional Bromophenol Blue
Suitable for agarose and non-denaturing polyacrylamide gel electrophoresis
Pre-mixed formulation for convenient sample preparation

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Daha fazla detay

ClearBand 6x DNA Loading Dye Green is a pre-mixed buffer designed for loading DNA markers and samples into the wells of agarose and non-denaturing polyacrylamide gels. The formulation contains two tracking dyes—Xylene Cyanol FF and Orange G—to visualize DNA migration across gel matrices. Orange G runs at approximately 50 bp in agarose gels, enabling superior tracking of DNA molecules before they migrate out of the gel. Unlike Bromophenol Blue, Orange G does not produce heavy background shadows on the gel, providing clear visualization of target DNA bands extending to approximately 400 bp, where PCR product resolution is typically required.

Technical Specifications

Primary Tracking Dye	Orange G
Secondary Tracking Dye	Xylene Cyanol FF
Detection Range	Up to ~400 bp
Compatible Matrices	Agarose gels, non-denaturing polyacrylamide gels

Applications

DNA marker and sample loading in routine molecular biology experiments
PCR product analysis and fragment size estimation
Visualization of DNA migration in agarose gel electrophoresis
Non-denaturing PAGE applications requiring real-time monitoring
Genomic DNA and plasmid DNA analysis
Restriction digest fragment analysis

Frequently Asked Questions

What is the advantage of Orange G over Bromophenol Blue in DNA loading dyes?

Orange G provides superior gel visualization by running at approximately 50 bp without creating heavy background shadows. Bromophenol Blue produces dark interference bands that can obscure DNA fragments in the 300-400 bp region. Orange G extends the visible tracking range to ~400 bp where PCR products typically appear.

What do the two tracking dyes (Orange G and Xylene Cyanol FF) indicate?

Orange G and Xylene Cyanol FF serve as separate migration markers. Orange G runs faster at ~50 bp, providing early front-loading visualization, while Xylene Cyanol FF migrates at a different rate, offering dual reference points for DNA tracking across the gel.

Is this dye compatible with both agarose and polyacrylamide gels?

Yes, ClearBand 6x DNA Loading Dye Green is compatible with both agarose gels and non-denaturing polyacrylamide gels.

How should this loading dye be used with DNA samples?

Mix DNA samples with ClearBand 6x DNA Loading Dye Green at appropriate ratios (typically 5-6:1 sample to dye ratio) before loading into gel wells. The pre-mixed formulation simplifies preparation.