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DNA Marker 1kb-Blue – Transparent Molecular Weight Standard

Fiyat Sorunuz

Out of stock

Ready-to-load molecular weight marker for precise DNA fragment size determination in agarose gel electrophoresis

  • 14 distinct bands spanning 250 bp to 25 kb range
  • Ready-to-load format – no preparation required
  • Enhanced intensity bands at 1000 and 3000 bp for rapid visual reference
  • Transparent formulation for optimal visualization
  • Approximate mass data provided per band at 5 μl loading volume
  • Suitable for both qualitative and quantitative gel analysis

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A transparent DNA Marker 1kb-Blue is a ready-to-load molecular weight standard designed for accurate determination of DNA fragment sizes during gel electrophoresis. This marker contains PCR products and double-stranded DNA, digested with appropriate restriction enzymes to generate 14 distinct bands ranging from 250 bp to 25 kb. The 1000 and 3000 base pair bands feature enhanced intensity to serve as prominent reference points for rapid orientation. Approximate mass values for each DNA band are provided based on a 5 μl loading volume, facilitating quantitative estimation of DNA mass in relatively concentrated DNA samples.

Technical Specifications

Band Range 250 bp – 25 kb
Number of Bands 14 bands
Reference Intensity Bands 1000 bp and 3000 bp
Standard Loading Volume 5 μl
DNA Type Double-stranded DNA (dsDNA) and PCR products
Format Transparent, ready-to-load

Applications

  • Molecular weight determination in agarose gel electrophoresis
  • PCR product size verification
  • Qualitative DNA fragment analysis
  • Quantitative DNA mass estimation in concentrated samples
  • Standard reference for molecular biology applications
  • Quality control in DNA purification and restriction enzyme digestion

Frequently Asked Questions

Is this marker ready to use directly?
Yes, this is a ready-to-load molecular weight marker. No preparation or dilution is required before loading into gel wells.
What is the purpose of the enhanced intensity bands at 1000 and 3000 bp?
These bands serve as prominent visual reference points for rapid orientation and quick size estimation during gel analysis.
Can this marker be used for quantitative DNA analysis?
Yes. Approximate mass values for each DNA band are provided based on a 5 μl loading volume, allowing estimation of DNA mass in relatively concentrated samples.
What gel electrophoresis format is this marker designed for?
This marker is specifically designed for agarose gel electrophoresis applications.
How many bands will I observe in a gel?
You will observe 14 distinct bands ranging from 250 bp to 25 kb in size.