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Dulbecco’s Modified Eagle Medium (DMEM) High Glucose

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Standard basal cell culture medium with high glucose concentration for diverse mammalian cell types

Four times higher amino acid and vitamin concentrations than original Eagle formulation
4500 mg/L glucose concentration for enhanced cellular metabolism
Supports diverse mammalian cell types including primary cells and established cell lines
Prepared from tissue culture grade water with 0.1 micron sterilization

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Daha fazla detay

Dulbecco's Modified Eagle Medium (DMEM) is a standard basal cell culture medium that represents a modification of Eagle's Basal Medium. NutriCulture DMEM High Glucose provides four times higher amino acid and vitamin concentrations compared to Eagle's Original Minimal Essential Medium. Originally formulated at 1000 mg/L glucose for embryonic mouse cell culture, it has been extensively modified to support growth of primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as established cell lines including HeLa, HEK 293, COS-7, and PC-12. Each formulation offers optimized L-glutamine and sodium pyruvate combinations, with glucose levels elevated to 4500 mg/L, reflecting the High Glucose designation. NutriCulture DMEM High Glucose is prepared from powdered basal medium and tissue culture grade water, then sterilized through 0.1 micron filtration. Every batch is verified for absence of bacterial, fungal, and mycoplasmal contamination.

Technical Specifications

Glucose Concentration	4500 mg/L
Original Glucose Level	1000 mg/L
Amino Acid Content	4x concentration vs. Eagle's Minimal Essential Medium
Vitamin Content	4x concentration vs. Eagle's Minimal Essential Medium
Sterilization Filter Size	0.1 micron
Source Material	Powdered basal medium + tissue culture grade water

Applications

Primary fibroblast culture
Neuron culture and differentiation
Glial cell culture
HUVEC (Human Umbilical Vein Endothelial Cell) culture
Smooth muscle cell culture
HeLa cell line culture
HEK 293 cell line culture
COS-7 cell line culture
PC-12 cell line culture
Embryonic mouse cell culture

Frequently Asked Questions

What is the main difference between standard DMEM and DMEM High Glucose?

DMEM High Glucose contains 4500 mg/L glucose compared to 1000 mg/L in the original formulation, supporting enhanced cellular metabolism and growth for various mammalian cell types.

Which cell types are compatible with NutriCulture DMEM High Glucose?

This medium supports primary cells (fibroblasts, neurons, glial cells, HUVECs, smooth muscle cells) and established cell lines (HeLa, HEK 293, COS-7, PC-12), with formulations offering optimized L-glutamine and sodium pyruvate combinations.

How is NutriCulture DMEM High Glucose prepared?

Each batch is prepared from powdered basal medium and tissue culture grade water, then sterilized through 0.1 micron filtration to ensure sterility and quality.