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NutriCulture Mycoplasma Detection Kit

Fiyat Sorunuz

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Sensitive and specific PCR-based detection system for mycoplasma contamination in cell cultures

Direct mycoplasma detection using PCR methodology
High sensitivity and specificity for reliable results
Integrated DNA extraction and purification protocol
Taq polymerase inhibitor exclusion system
Complete internal, positive, and negative control reactions
Rapid turnaround time for screening
Cost-effective solution for high-throughput sample screening
Suitable for routine monitoring of cell cultures

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Daha fazla detay

Mycoplasma contamination in cell cultures represents a significant challenge in routine cell culture operations. While bacterial, fungal, and yeast contamination are readily detected through culture turbidity changes and inverted microscope visualization, mycoplasma—a bacterium belonging to the Mollicutes class—frequently evades detection. This organism can persist undetected for extended periods in cell cultures without producing visible cytopathic effects. NutriCulture Mycoplasma Detection Kit provides a sensitive and specific alternative using polymerase chain reaction (PCR) technology for direct mycoplasma detection in cell cultures. The kit includes integrated DNA extraction and purification from cultured cells to exclude Taq polymerase inhibitors that may be present in raw cell culture solutions, even after serial dilution. All essential control reactions—internal, positive, and negative controls—are included.

Technical Specifications

Detection Method	Polymerase Chain Reaction (PCR)
Target Organism	Mycoplasma (Mollicutes class)
Sample Type	Cell culture supernatant and pellet
Processing Step	DNA extraction and purification included

Applications

Routine screening of newly acquired cell lines entering the laboratory
Initial quality control analysis of primary cell cultures
Periodic monitoring of actively maintained cell culture stocks
High-throughput contamination screening of multiple samples
Validation of cell culture authenticity and integrity
Pre-expansion mycoplasma screening protocols

Frequently Asked Questions

Why is mycoplasma detection important in cell culture?

Mycoplasma can persist undetected in cell cultures without producing visible cytopathic effects. This cryptic contamination compromises research integrity and can invalidate experimental results. Routine detection is essential for maintaining cell culture quality.

Why doesn't routine microscopic examination detect mycoplasma?

Mycoplasma, belonging to the Mollicutes class, does not produce visible changes in culture turbidity or morphological alterations visible under inverted microscopy, making them difficult to detect by conventional methods.

What advantages does PCR offer over other detection methods?

PCR provides superior sensitivity, high specificity, rapid results, cost-effectiveness, and the ability to screen multiple samples simultaneously, making it ideal for routine laboratory applications.

Why is DNA extraction and purification necessary?

Raw cell culture solutions often contain Taq polymerase inhibitors that can compromise PCR reactions. The integrated extraction and purification step eliminates these inhibitors, even when serial dilution proves insufficient.

What controls are included in the kit?

The kit includes internal controls to monitor reaction efficiency, positive controls to confirm assay functionality, and negative controls to detect false positives or contamination.