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NutriCulture RPMI 1640 Medium

Fiyat Sorunuz

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Serum-free mammalian cell culture medium formulated for suspension and monolayer cultivation

Formulated for 5% CO2 atmosphere
Suitable for suspension and monolayer culture systems
Validated for multiple mammalian cell line cultivation
Serum-free formulation for lymphoid cell propagation
Tissue culture-grade quality
Sterile filtration through 0.1 micron membrane

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Daha fazla detay

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Daha fazla detay

NutriCulture RPMI 1640, originally developed in 1966 by Moore and colleagues at Roswell Park Memorial Institute, is a chemically defined medium formulated for culturing human leukemia cells in both suspension and monolayer configurations. Optimized for use in 5% CO2 atmosphere, it has been validated for culture of diverse mammalian cell lines including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. NutriCulture RPMI 1640 is traditionally employed for serum-free proliferation of human lymphoid cells in karyotype analysis applications.

Technical Specifications

Product Form	Powder base medium
Preparation	Tissue culture-grade water
Sterilization Method	Membrane filtration (0.1 micron)
CO2 Requirement	5%

Applications

Human leukemia cell culture
Mammalian cell line maintenance
HeLa, Jurkat, MCF-7, PC12, PBMC cultivation
Astrocyte and carcinoma cell culture
Human lymphoid cell serum-free propagation
Karyotype analysis applications
Suspension and monolayer culture systems

Frequently Asked Questions

What is the historical background of RPMI 1640?

RPMI 1640 was originally developed in 1966 by Moore and colleagues at Roswell Park Memorial Institute for culturing human leukemia cells in suspension and monolayer.

What cell lines are compatible with NutriCulture RPMI 1640?

The medium is validated for culture of HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and various carcinoma cell lines.

What atmospheric conditions does this medium require?

NutriCulture RPMI 1640 is formulated for use in 5% CO2 atmosphere.

How is each batch prepared?

Each batch is prepared from powder base medium using tissue culture-grade water and sterilized through 0.1 micron membrane filtration.

What quality assurance testing is performed?

Every batch is tested to confirm the absence of bacterial, fungal, and mycoplasmal contamination.