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Trypsin-EDTA (0.25%) – Cell Culture Dissociation Solution

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A 0.25% concentration trypsin-EDTA solution used in passaging adherent cell cultures. Suitable for cell biology laboratories.

0.25% trypsin concentration
EDTA-containing formulation (calcium/magnesium chelation)
Standard use for adherent cell passaging
Designed for cell culture applications
Pre-warming to 37°C recommended before use

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Daha fazla detay

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Daha fazla detay

Trypsin-EDTA (0.25%) is a standard dissociation solution used for detaching and passaging adherent cell cultures from flasks or plates. Trypsin proteolytically cleaves cell-surface and cell-cell adhesion proteins, while EDTA chelates divalent cations (Ca2+, Mg2+), disrupting the function of cell adhesion molecules and facilitating easier cell detachment from the surface.

In cell culture laboratories, it is routinely used for passaging, dissociation prior to cell counting, and experimental preparation. Prior to use, the solution is typically warmed to 37°C, and trypsin activity should be neutralized with serum-containing medium following the procedure.

It is expected to be a sterile reagent manufactured to cell culture grade quality; laboratories are advised to determine optimal incubation time with their own cell lines.

Technical Specifications

Trypsin Concentration	0.25%
Composition	Trypsin-EDTA
SKU	TE025
Application Area	Cell culture, cell dissociation

Applications

Passaging of adherent cell lines
Cell dissociation prior to cell counting
Routine cell culture maintenance

Frequently Asked Questions

Why should trypsin-EDTA be warmed before use?

Trypsin exhibits optimal enzymatic activity around 37°C; pre-warming the solution before applying to cells enhances dissociation efficiency.

What should be done following trypsin treatment?

To prevent cell damage, trypsin activity must be neutralized with serum-containing medium, and cells should be resuspended in fresh medium after centrifugation.

Which cell types is 0.25% trypsin suitable for?

It is suitable for most standard adherent cell lines; however, concentration and incubation time should be optimized for sensitive cell lines.