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ClearBand TRUEzol Reagent

Fiyat Sorunuz

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Ready-to-use phenol-based reagent for total RNA isolation from diverse biological samples

High-quality total RNA isolation from multiple biological sources
Ready-to-use formulation - no preparation required
Efficient three-phase separation for superior purity
Phenol-based extraction chemistry for optimal RNA recovery
Compatible with diverse downstream applications
Clear phase separation for reliable visual assessment

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Daha fazla detay

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Daha fazla detay

ClearBand TRUEzol Reagent is a ready-to-use formulation comprising phenol and other optimized components designed for high-yield isolation of total RNA from a wide range of biological materials, including animal tissues, plant tissues, cells, and bacterial cultures. The reagent enables efficient extraction through controlled phase separation, yielding pure, intact RNA suitable for downstream molecular applications.

Technical Specifications

Composition	Phenol and proprietary buffered components
Application	Total RNA isolation
Suitable Sample Types	Animal tissues, plant tissues, cells, bacterial cultures
Phase Separation	Three-phase system (aqueous, organic, interphase)
Purification Method	Isopropanol precipitation and washing

Applications

cDNA synthesis and reverse transcription
Northern blot analysis
Differential display PCR
Primer extension analysis
mRNA selection and enrichment
Gene expression profiling
RNA-seq sample preparation
RT-qPCR template preparation

Frequently Asked Questions

What are the main steps in the ClearBand TRUEzol extraction protocol?

Biological samples are homogenized or lysed in ClearBand TRUEzol Reagent, followed by three-phase separation. The clear upper aqueous phase contains RNA, the pink lower organic phase contains lipids, and the interphase contains DNA and proteins. RNA is purified by isopropanol precipitation and subsequent washing steps.

How can I visually confirm proper phase separation?

Successful extraction produces three distinct phases: a clear upper aqueous phase (containing RNA), a pink lower organic phase, and a white interphase layer (containing DNA and proteins). Clear phase demarcation indicates optimal extraction conditions.

Which downstream applications are compatible with isolated RNA?

ClearBand TRUEzol-purified RNA is suitable for cDNA synthesis, northern blotting, differential display, primer extension, mRNA selection, RT-qPCR, microarray analysis, and RNA sequencing applications.

Is ClearBand TRUEzol suitable for difficult sample types?

The reagent is effective for diverse biological sources including animal tissues, plant tissues, cultured cells, and bacterial samples. Extended homogenization may be required for challenging matrices.