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Novatek FFPE Total RNA Kit (Eco Spin) – E2095

Fiyat Sorunuz

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Total RNA purification kit from formalin-fixed, paraffin-embedded (FFPE) tissues. Does not require hazardous chemicals such as phenol-chloroform; delivers results in less than 40 minutes.

  • Total RNA purification from FFPE (formalin-fixed, paraffin-embedded) tissues
  • Silica-based membrane and chaotropic ion technology
  • No requirement for phenol-chloroform, β-mercaptoethanol, or alcohol precipitation
  • Room temperature protocol completed in less than 40 minutes
  • Purified RNA suitable for routine downstream applications (RT-PCR, etc.)

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The Novatek FFPE Total RNA Kit is specifically designed for total RNA isolation from formalin-fixed and paraffin-embedded tissue specimens. It provides a practical solution for researchers working with pathology archive samples and clinical tissue banks.

The kit is based on chaotropic ions and silica-based membrane technology, eliminating the need for expensive resins, phenol-chloroform extraction, β-mercaptoethanol use, or time-consuming alcohol precipitation steps. The standard protocol is performed at room temperature with a total processing time under 40 minutes.

The purified RNA is suitable for routine downstream applications (e.g., RT-PCR, gene expression analysis). The simplified workflow ensures a low learning curve for laboratory personnel and reduces processing time per sample.

Technical Specifications

Sample Type FFPE (formalin-fixed, paraffin-embedded) tissue
Isolation Technology Silica-based membrane / chaotropic ion
Protocol Duration < 40 minutes
Operating Temperature Room temperature
Hazardous Chemical Requirement None (phenol-chloroform and β-mercaptoethanol not required)
Kit Code (SKU) E2095

Applications

  • Total RNA isolation from pathology archive FFPE tissue specimens
  • Gene expression studies with clinical tissue bank samples
  • Total RNA preparation prior to RT-PCR

Frequently Asked Questions

Which sample types can be used with this kit?
The kit is designed for total RNA isolation from formalin-fixed, paraffin-embedded (FFPE) tissue specimens.
Are hazardous chemicals such as phenol-chloroform required?
No. The kit utilizes silica membrane and chaotropic ion technology, eliminating the need for such hazardous extraction steps.
How long does the protocol take?
The standard protocol operates at room temperature and is completed in less than 40 minutes.