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Eco Spin Plasmid Isolation Kit

Fiyat Sorunuz

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High-yield plasmid DNA purification from recombinant E. coli using silica-based membrane technology

Rapid purification in <25 minutes
High yield up to 20 μg plasmid DNA per extraction
Chaotropic salt and silica-based membrane technology
Eliminates ethanol precipitation
Compatible with all plasmid and cosmid sizes
Cost-effective purification method

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Daha fazla detay

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Daha fazla detay

Eco Spin Plasmid Isolation Kit is designed for simple, convenient, and cost-effective purification of high-quality plasmid DNA from recombinant E. coli cultures. Utilizing chaotropic ions and silica-based membrane technology, this kit eliminates the need for time-consuming ethanol precipitation methods. The standard protocol is completed in less than 25 minutes and yields up to 20 μg of plasmid DNA. The kit is effective for purification of plasmids and cosmids of all sizes. Relative plasmid yield and optimal culture size depend on the plasmid copy number used in the bacterial culture and the growth medium employed.

Technical Specifications

Protocol Duration	<25 minutes
Maximum DNA Yield	up to 20 μg per extraction
Purification Technology	Chaotropic ions + silica-based membrane
Compatible Insert Size	All plasmid and cosmid sizes
Source Material	Recombinant E. coli cultures

Applications

Rapid plasmid DNA extraction from bacterial cultures
Cosmid purification for genomic library applications
High-throughput plasmid preparation workflows
Molecular cloning and transformation studies
Plasmid quality control and analysis

Frequently Asked Questions

Can this kit purify cosmids?

Yes, the Eco Spin Kit is effective for purification of plasmids and cosmids of all sizes.

Why is ethanol precipitation eliminated?

The chaotropic ion and silica-based membrane technology directly binds and elutes DNA without requiring ethanol precipitation, significantly reducing protocol time.

How does plasmid copy number affect yield?

Relative plasmid yield is directly dependent on the plasmid copy number present in your E. coli culture and the specific growth medium composition.