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SENSISpec ELISA Lupin Allergen 48-Well Kit

Fiyat Sorunuz

High-sensitivity ELISA kit for detection and quantification of lupin allergen residues in food products

  • Sandwich enzyme immunoassay (ELISA) format
  • Quantitation range: 2-30 ppm
  • Low limits of detection: 0.1-0.7 ppm depending on food matrix
  • High cross-reactivity with Lupinus albus, L. angustifolius, and L. luteus
  • Minimal cross-reactivity with other legumes (chickpea 0.0003%, lentil 0.0004%)
  • 450 nm optical detection (HRP + TMB substrate)
  • Total incubation time: 60 minutes (20+20+20 min protocol)
  • Microtitre plate format for 48-well configuration
  • 13-month shelf life at 2-8°C

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The SENSISpec Lupin ELISA is a sandwich enzyme immunoassay providing highly sensitive detection of lupin allergen residues in food matrices including sausages, baked goods, potato products, ketchup, and fruit juices. Lupin (Lupinus species: L. albus, L. angustifolius, L. luteus) is a legume with protein content of 35-45% in seeds, containing allergenic proteins (α-, β-, γ-conglutin fractions) with documented cross-reactivity to soybean and peanut allergens. Due to increasing use of lupin flour in food manufacturing—particularly in products for consumers with milk allergy, vegetarian diets, and celiac disease—detection of hidden lupin allergen residues is critical for food safety. Cross-contamination during production is common, and even trace amounts can trigger severe allergic reactions including anaphylaxis. This assay demonstrates high cross-reactivity with common lupin varieties and provides sensitive quantification for allergen control and regulatory compliance.

Technical Specifications

SKU HU0030035
Test Principle Sandwich enzyme immunoassay
Format Microtitre plate (48-well)
Total Incubation Time 60 minutes (20/20/20 min protocol)
Standard Concentrations 0; 2.5; 15; 30 ppm
Optical Detection 450 nm (HRP + TMB substrate)
Limit of Detection (LOD) Sausage 0.2 ppm, Bread 0.3 ppm, Orange juice 0.7 ppm, Ketchup 0.1 ppm, Croquettes 0.2 ppm
Quantitation Range 2-30 ppm
Cross-Reactivity Chickpea 0.0003%, Lentil 0.0004%, Soya flour (unroasted) 0.07%, Soya flour (roasted) 0.0009%
Shelf Life 13 months
Storage Temperature 2-8°C

Applications

  • Detection and quantification of lupin allergen residues in food products for regulatory compliance
  • Quality control and allergen monitoring in sausage and processed meat manufacturing
  • Lupin residue testing in baked goods and cereal-based products
  • Allergen verification in potato-based products and croquettes
  • Lupin detection in condiments including ketchup and sauces
  • Allergen screening in fruit juices and beverage products
  • Cross-contamination monitoring in multi-allergen food production facilities
  • Food safety validation for products targeting consumers with peanut/soybean allergies
  • Compliance testing for allergen labeling and declaration requirements

Frequently Asked Questions

What is lupin and why is allergen testing necessary?
Lupin (Lupinus species) is a legume increasingly used in food manufacturing as a protein-rich flour substitute, particularly in products for dietary-restricted consumers. With protein content of 35-45% containing allergenic conglutin fractions, lupin poses significant allergen risk. Even trace amounts can trigger severe reactions including anaphylaxis in sensitized individuals.
Does this assay detect all common lupin varieties?
Yes. The SENSISpec Lupin ELISA demonstrates high cross-reactivity with the three principal lupin species used in food production: Lupinus albus, Lupinus angustifolius, and Lupinus luteus.
What are the limits of detection for different food matrices?
LOD varies by food matrix: ketchup 0.1 ppm (most sensitive), sausage 0.2 ppm, croquettes 0.2 ppm, bread 0.3 ppm, and orange juice 0.7 ppm. The quantitation range is 2-30 ppm for all matrices.
Does lupin cross-react with other allergens?
Lupin contains conglutin proteins with documented cross-reactivity to soybean and peanut allergens. However, this assay shows minimal cross-reactivity with other legumes: chickpea 0.0003%, lentil 0.0004%, and roasted soya flour 0.0009%.
What is the incubation protocol?
Total assay time is 60 minutes using a 20/20/20 minute three-step incubation protocol, providing efficient high-throughput screening capability.
How should the kit be stored?
Store at 2-8°C. The kit maintains stability for 13 months under proper storage conditions.
What detection method is used?
The assay uses sandwich ELISA with horseradish peroxidase (HRP) enzyme and TMB chromogenic substrate, with optical detection at 450 nm wavelength.