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ClearBand 6x DNA Loading Dye (Blue) – DLD-6xB

Fiyat Sorunuz

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Pre-mixed 6x loading buffer for loading DNA samples into agarose and non-denaturing polyacrylamide gels. Dual tracking dyes enable visual monitoring of gel migration.

  • 6x concentrated, pre-mixed ready-to-use buffer
  • Dual tracking dyes: Xylene Cyanol FF and Bromophenol Blue
  • Migration at approximately 400 bp DNA fragment level in agarose gels (both dyes)
  • Compatible with agarose and non-denaturing polyacrylamide gels
  • Easy-to-use for DNA sample and marker loading

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ClearBand 6x DNA Loading Dye is a ready-to-use, 6x concentrated buffer formulated for loading DNA samples and markers into wells of agarose or non-denaturing polyacrylamide gels. This product meets routine electrophoresis needs in every molecular biology laboratory, simplifying the sample loading process and enabling visual tracking of DNA migration within the gel.

The two tracking dyes present in this formulation migrate at different rates: Xylene Cyanol FF travels approximately at the level of a 400 bp DNA fragment in agarose gels, while Bromophenol Blue migrates at approximately 400 bp in agarose gels. This dual-dye system ensures timely termination of electrophoresis before samples run out of the gel and are lost.

With the 6x formulation, samples can be easily mixed in simple ratios (e.g., 5 units sample + 1 unit dye) without requiring separate preparation steps. The dye is suitable for standard agarose gel electrophoresis and non-denaturing PAGE applications.

Technical Specifications

Manufacturer ClearBand
Concentration 6x
Tracking Dyes Xylene Cyanol FF, Bromophenol Blue
Compatible Gel Types Agarose, non-denaturing polyacrylamide (PAGE)
Color Blue
Product Code DLD-6xB

Applications

  • DNA sample and marker loading in agarose gel electrophoresis
  • Non-denaturing polyacrylamide gel electrophoresis (PAGE)
  • Visual tracking of DNA fragment separation

Frequently Asked Questions

How is the 6x DNA Loading Dye used?
Mix with samples at approximately a 5:1 ratio (5 units sample, 1 unit dye) and load into gel wells. No separate preparation is required.
Which gel types are compatible?
The dye can be used with agarose gels and non-denaturing polyacrylamide gels.
What do the tracking dyes indicate?
Xylene Cyanol FF and Bromophenol Blue visualize DNA migration within the gel at approximately the 400 bp DNA fragment level, allowing timely termination of electrophoresis.