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ClearBand TAE 50x Electrophoresis Buffer

Fiyat Sorunuz

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Concentrated TAE electrophoresis buffer for high-resolution nucleic acid separation in agarose and polyacrylamide gel applications.

50x concentrated stock solution for convenient preparation
Filter sterilized and prepared with ultrapure water
Optimized for high-resolution agarose gel electrophoresis
Suitable for high molecular weight double-stranded DNA separation
Requires dilution to 1x working concentration before use

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Daha fazla detay

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Daha fazla detay

ClearBand TAE 50x Electrophoresis Buffer is a concentrated running buffer widely used for the separation of nucleic acids in agarose and polyacrylamide gels. Specifically formulated for high-resolution agarose gel electrophoresis applications and preferred for the separation of high molecular weight double-stranded DNA. The buffer must be diluted to 1x working concentration before use. Fresh 1X buffer is recommended for each electrophoresis run to ensure optimal separation and resolution.

Technical Specifications

Concentration	50x (concentrated stock)
Working Concentration	1x
1x Buffer Composition	40 mM Tris, 20 mM acetic acid, 1 mM EDTA
Sterilization	Filter sterilized
Preparation	Ultrapure water

Applications

Agarose gel electrophoresis
Polyacrylamide gel electrophoresis (PAGE)
High-resolution DNA separation
Double-stranded DNA fragment analysis
High molecular weight nucleic acid separation
Genomic DNA analysis

Frequently Asked Questions

What is the proper dilution ratio for ClearBand TAE 50x Buffer?

Dilute the 50x concentrate to 1x working concentration by adding 1 part buffer to 49 parts ultrapure water.

Should buffer be reused between electrophoresis runs?

No, fresh 1X buffer should be used for each electrophoresis application to maintain optimal separation quality and reduce artifacts.

What applications is this buffer suitable for?

ClearBand TAE 50x is specifically formulated for high-resolution agarose gel electrophoresis and separation of high molecular weight double-stranded DNA in both agarose and polyacrylamide gels.

How should this concentrated buffer be stored?

Store the 50x concentrated stock at room temperature away from direct sunlight. Preparation method ensures sterility through filter sterilization with ultrapure water.