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Pre-stained Protein Marker, 10-245 kDa

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Multi-color pre-stained protein standard with 12 protein bands for SDS-PAGE analysis and Western blot transfer verification

12 pre-stained protein bands for comprehensive molecular weight reference
Wide range coverage: 10-245 kDa
Two distinct reference bands (25 kDa green, 75 kDa red) for orientation
Blue chromophore for gel visualization
Compatible with SDS-PAGE (Tris-glycine buffer systems)
Ready-to-use format in gel loading buffer
Enables real-time monitoring during electrophoresis
Verify Western blot transfer efficiency

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Daha fazla detay

A comprehensive protein standard containing 12 pre-stained proteins spanning a broad molecular weight range from 10 to 245 kDa. The proteins are covalently coupled with a blue chromophore and include two reference bands: a green band at 25 kDa and a red band at 75 kDa when separated on SDS-PAGE (Tris-glycine buffer). This marker is designed to monitor protein separation during SDS-polyacrylamide gel electrophoresis, verify Western blot transfer efficiency onto membranes, and estimate protein molecular weight. The product is supplied in gel loading buffer and is ready to use directly.

Technical Specifications

Molecular Weight Range	10-245 kDa
Number of Protein Bands	12
Reference Band 1	25 kDa (Green)
Reference Band 2	75 kDa (Red)
Chromophore Color	Blue
Buffer System Compatibility	Tris-glycine buffer
Format	Ready-to-use in gel loading buffer

Applications

Real-time monitoring of protein separation during SDS-PAGE
Molecular weight estimation of unknown proteins
Verification of Western blot transfer efficiency
Quality control in protein analysis workflows
Gels and gel staining standardization
Troubleshooting electrophoresis procedures

Frequently Asked Questions

What buffer system is this marker compatible with?

This pre-stained protein marker is designed for use with Tris-glycine buffer systems in SDS-PAGE applications.

Are there reference bands for orientation?

Yes, the marker contains two reference bands: a green band at 25 kDa and a red band at 75 kDa, which serve as orientation markers during gel analysis.

Is this product ready to use?

Yes, the marker is supplied in gel loading buffer and is ready to use directly without additional preparation.

Can this marker be used for Western blot transfer verification?

Yes, one of the primary applications is verifying the transfer efficiency of proteins onto membranes during Western blotting procedures.

How many protein bands does this marker contain?

The marker contains 12 pre-stained protein bands covering the molecular weight range of 10-245 kDa.