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SENSISpec ELISA Cashew Allergen 96-Well

Fiyat Sorunuz

Sensitive sandwich immunoassay kit for cashew protein detection in food matrices with 0.2 ppm LOD

  • Highly sensitive sandwich ELISA detection method
  • Quantitation range: 2–60 ppm
  • Limit of detection: 0.2 ppm
  • Heat-stable allergen markers
  • Suitable for processed food analysis (cookies, cereals, ice cream, chocolate)
  • Minimal cross-reactivity (4% peanut cross-reactivity)
  • Ready-to-use 96-well microtiter plate format
  • 13-month shelf life at 2–8°C

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SENSISpec Cashew ELISA represents a highly sensitive detection system specifically designed for the quantification of cashew allergen residues in food products. Cashew kernels contain approximately 18% protein, with several well-characterized allergens including vicilin Ana o 1, legumin Ana o 2, and albumin Ana o 3. These allergens are predominantly heat-stable, maintaining their allergenic properties across various processing conditions, making cashew a significant food allergen concern. Cross-contamination during manufacturing is frequent, often rendering the exclusion of cashew residues in food products impossible. This ELISA kit provides robust detection capabilities for cashew residues in complex food matrices such as cookies, cereals, ice cream, and chocolate products.

Technical Specifications

SKU HU0030004
Test Principle Sandwich enzyme immunoassay
Format Microtiter plate
Incubation Time 60 min (20/20/20 min)
Standard Concentrations 0; 2; 6; 20; 60 ppm
Detection Wavelength 450 nm (HRP + TMB)
Limit of Detection (LOD) 0.2 ppm
Quantitation Range 2–60 ppm
Cross-Reactivity Peanut 4%
Shelf Life 13 months
Storage Condition 2–8°C

Applications

  • Cashew allergen detection in confectionery products
  • Residual allergen analysis in processed nuts and seeds
  • Cashew contamination screening in cereal products
  • Ice cream and frozen dessert allergen quantification
  • Chocolate product allergen compliance testing
  • Cross-contamination risk assessment in food manufacturing
  • Regulatory compliance monitoring for food labeling

Frequently Asked Questions

What makes cashew allergens particularly problematic?
Cashew proteins (~18% of kernel weight) include heat-stable allergens such as Ana o 1 (vicilin), Ana o 2 (legumin), and Ana o 3 (albumin). Their thermal stability allows them to survive food processing, and even trace amounts can trigger anaphylactic reactions in sensitized individuals.
What is the detection limit of this ELISA kit?
The limit of detection (LOD) is 0.2 ppm, enabling reliable quantification of cashew allergen residues at very low concentrations.
What is the cross-reactivity profile?
The kit shows 4% cross-reactivity with peanut, which is acceptable for cashew-specific allergen detection while maintaining good specificity.
What food products can be tested with this kit?
The ELISA is specifically optimized for cashew residue analysis in cookies, cereals, ice cream, chocolate, and other processed food matrices.
How should the kit be stored?
Store at 2–8°C (refrigerated conditions) for optimal stability. The kit maintains its integrity for 13 months under these conditions.
What is the total assay time?
The total incubation time is 60 minutes, structured as 20/20/20 minutes for sequential incubation steps, allowing efficient workflow integration.