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SENSISpec Fish (Parvalbumin) ELISA Kit 96-Well Allergen Test

Fiyat Sorunuz

High-sensitivity sandwich ELISA kit for specific detection of fish allergen (parvalbumin) residues in food matrices with 1.4 ppm limit of detection

  • Trans-species parvalbumin detection with high specificity
  • Sandwich enzyme immunoassay format (EIA/ELISA)
  • Limit of Detection: 1.4 ppm for reliable low-level quantification
  • Quantification range: 4–100 ppm with five calibration standards
  • 60-minute total incubation protocol (20/20/20 min distributed)
  • Microplate format enabling batch sample processing
  • HRP-TMB detection system at 450 nm optical wavelength
  • No cross-reactivity with non-target fish species or allergens
  • Extended shelf life of 24 months under standard storage conditions
  • Temperature-stable protein quantification applicable to thermally processed matrices

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SENSISpec Fish ELISA Kit represents a highly sensitive detection system based on trans-species fish allergen parvalbumin, specifically engineered to quantify fish residues in wines, soups, sauces, crackers, surimi, and similar processed food products. Fish ranks among the most frequent food allergen triggers. Allergic reactions are typically induced by parvalbumin, a low-molecular-weight, calcium-binding muscle protein characterized by high thermal resistance, stability against denaturing agents, and resilience to proteolytic enzymes. In regions with high fish consumption—including Scandinavia, Japan, Mediterranean countries, and Asia-Pacific regions—fish allergies represent a significant public health concern. Clinical symptoms vary from dermal inflammation to gastrointestinal and respiratory complications, extending to life-threatening anaphylactic shock. High cross-reactivity among fish species necessitates comprehensive allergen screening protocols.

Technical Specifications

Stock Code HU0030008
Test Principle Sandwich enzyme immunoassay
Format Microtitre plate
Incubation Duration 60 minutes (20/20/20 minutes)
Calibration Standards 0; 4; 10; 40; 100 ppm
Detection Wavelength 450 nm (HRP + TMB)
Limit of Detection (LOD) 1.4 ppm
Quantification Range 4–100 ppm
Cross-Reactivity None
Shelf Life 24 months
Storage Conditions 2–8°C

Applications

  • Fish allergen screening in processed seafood products and surimi-based formulations
  • Regulatory compliance testing for food labeling and allergen declaration requirements
  • Quality assurance in multi-ingredient food manufacturing (sauces, dressings, broths)
  • Cross-contamination monitoring in seafood processing facilities
  • Wine and vinegar production allergen verification protocols
  • Crackers and baked goods with fish-derived ingredients or processing aids
  • HACCP-based allergen control programs in food manufacturing
  • Certification testing for allergen-free product claims
  • Research applications in allergen immunochemistry and food safety
  • Third-party laboratory allergen testing and verification services

Frequently Asked Questions

Why is parvalbumin-based detection superior to total fish protein quantification?
Parvalbumin is the primary fish allergen responsible for clinical hypersensitivity reactions. Unlike non-allergenic fish proteins, parvalbumin maintains structural integrity during thermal processing, cooking, and enzymatic degradation, making it the most reliable biomarker for allergen risk assessment in processed foods.
Does this kit detect all fish species equally?
The parvalbumin epitopes targeted by this kit exhibit minimal cross-reactivity variation among fish species due to trans-species allergen homology. Detection efficiency is consistent across teleost fish, though species-specific parvalbumin levels may vary slightly.
How does the 1.4 ppm LOD compare to regulatory thresholds?
The 1.4 ppm LOD provides sensitive quantification well below typical labeling thresholds in EU (0.5% = 5000 ppm for declarable allergen) and precautionary-based contamination limits, enabling detection of accidental cross-contamination.
Are controls and standards included in the kit?
Yes. Five calibration standards (0, 4, 10, 40, 100 ppm) are pre-formulated. Positive and negative control materials are supplied. Storage at 2–8°C maintains standard stability throughout the 24-month shelf life.