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Shellfish (Tropomyosin) ELISA Kit SENSISpec 96-Well Allergen Test

Fiyat Sorunuz

High-sensitivity sandwich ELISA for tropomyosin detection in food matrices; 96-well microplate format with 0.9–10.3 ppb LOD range

  • Sandwich enzyme immunoassay principle for superior specificity
  • Microplate-based format enabling batch sample analysis
  • Multiple food matrix validation: soy sauce, vegetable soup, baked goods, fish, and meat products
  • Rapid incubation protocol: 60 minutes total (20/20/20 minute intervals)
  • Quantification range: 20–400 ppb with matrix-specific LOD from 0.9–10.3 ppb
  • HRP + TMB detection at 450 nm for reliable colorimetric readout
  • No cross-reactivity with other common allergens
  • 24-month shelf life under standard refrigerated storage (2–8°C)

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The SENSISpec Shellfish (Tropomyosin) ELISA Kit represents a highly sensitive detection system for tropomyosin (from Penaeus indicus) with proven capacity to quantify shellfish residues in fish products, broths, sauces, baked goods, and meat products. Shellfish allergens are a major food allergen group with documented cross-reactivity to house dust mites. Tropomyosin, the most important allergenic protein in common shellfish species, comprises approximately 20% of total protein in cooked shellfish extracts. Cross-contamination during manufacturing is frequently identified, making accurate detection of shellfish residues in food matrices essential for allergen management and food safety compliance.

Technical Specifications

Stock Code HU0030006
Test Principle Sandwich enzyme immunoassay
Format Microplate (96-well)
Incubation Time 60 minutes (20/20/20 minute intervals)
Calibration Standards 0, 20, 60, 200, 400 ppb
Detection Wavelength 450 nm (HRP + TMB)
Limit of Detection (LOD) Soy sauce: 1.7 ppb; Vegetable soup: 3.6 ppb; Baked goods: 0.9 ppb; Fish: 8.5 ppb; Meat: 10.3 ppb
Quantification Range 20–400 ppb
Cross-Reactivity None
Shelf Life 24 months
Storage Temperature 2–8°C

Applications

  • Food allergen detection in processed shellfish products
  • Shellfish residue quantification in seafood sauces and broths
  • Cross-contamination monitoring in multi-allergen manufacturing facilities
  • Regulatory compliance testing for food labeling and safety
  • Clinical research on shellfish allergen exposure and tolerance
  • Quality assurance in baked goods and meat product lines
  • Residue analysis in fish-based prepared foods

Frequently Asked Questions

What protein target does this kit detect?
The SENSISpec kit specifically detects tropomyosin from shellfish (Penaeus indicus). Tropomyosin is the primary allergenic protein in shellfish species, representing ~20% of total protein in cooked shellfish extracts.
What is the detection limit for different food matrices?
Limits of detection vary by matrix: soy sauce (1.7 ppb), vegetable soup (3.6 ppb), baked goods (0.9 ppb), fish (8.5 ppb), and meat products (10.3 ppb). The quantification range is 20–400 ppb.
Does the kit show cross-reactivity with other allergens?
No, the SENSISpec kit exhibits no cross-reactivity with other common allergens, ensuring allergen-specific detection.
How long does the assay take?
Total assay time is 60 minutes, consisting of three sequential 20-minute incubation periods followed by plate reading.
What storage conditions are required?
The kit must be stored at 2–8°C (refrigerated conditions) and maintains stability for 24 months under these conditions.
Is this kit suitable for regulatory testing?
Yes, the SENSISpec kit is designed for food safety and regulatory compliance applications, with validated performance across multiple food matrices commonly requiring allergen testing.