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SENSISpec Walnut ELISA Kit (48 Wells) – HU0030048

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Quantitative detection of walnut residues in food samples. ELISA kit with 0.35 ppm detection limit and 2-60 ppm measurement range, suitable for food allergen analysis laboratories.

  • 48-well microtiter plate format
  • Detection limit: 0.35 ppm
  • Quantification range: 2-60 ppm
  • Total incubation time 60 minutes (20/20/20)
  • Colorimetric detection at 450 nm with HRP+TMB
  • Detailed cross-reactivity profile (hazelnut, chestnut, pecan nut, etc.)

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SENSISpec Walnut ELISA Kit is a sandwich ELISA-format test kit designed for quantitative detection of walnut residues in processed food products (cookies, cereals, ice cream, chocolate, etc.). Manufactured by Gold Standard Diagnostics and supplied in 48-well microtiter plate format.

The kit provides rapid results with a total incubation time of 60 minutes (3×20 minutes); detection is performed colorimetrically at 450 nm using the HRP-TMB system. Calibration is provided with standards at 0; 2; 6; 20; 60 ppm, with a detection limit of 0.35 ppm and a quantification range of 2-60 ppm.

The kit's cross-reactivity profile is particularly important: <0.003% cross-reactivity is observed with soybean, pine nut, peanut, sesame, and Brazil nut, while hazelnut shows 0.022%, chestnut 0.11%, and pecan nut 0.80% cross-reactivity. This profile should be considered when evaluating false positive risk in walnut analysis.

Suitable for food safety laboratories, production facility quality control units, and laboratories providing allergen testing services. Shelf life is 13 months; store at 2-8°C.

Technical Specifications

Manufacturer Gold Standard Diagnostics
Product Code HU0030048
Allergen Type Walnut
Test Principle Sandwich ELISA
Format 48-well microtiter plate
Incubation Time 60 minutes (20/20/20 minutes)
Standards 0; 2; 6; 20; 60 ppm
Detection 450 nm (HRP + TMB)
Detection Limit 0.35 ppm
Quantification Range 2 – 60 ppm
Cross-Reactivity Soybean <0.003%, Pine nut <0.003%, Peanut <0.003%, Sesame <0.003%, Brazil nut <0.003%, Hazelnut 0.022%, Chestnut 0.11%, Pecan nut 0.80%
Shelf Life 13 Months
Storage 2-8°C

Applications

  • Walnut residue analysis in processed food products (cookies, cereals, ice cream, chocolate)
  • Allergen cross-contamination control in production facilities
  • Food labeling compliance testing
  • Routine analysis in allergen testing service laboratories

Frequently Asked Questions

What cross-reactivity levels does the kit show?
The lowest cross-reactivity (<0.003%) is observed with soybean, pine nut, peanut, sesame, and Brazil nut. The highest cross-reactivity is reported with pecan nut at 0.80%.
How long does it take to get results?
Total incubation time is 60 minutes using a three-stage protocol (20/20/20 minutes).
What is the kit's detection limit?
The kit operates with a detection limit of 0.35 ppm and a quantification range of 2-60 ppm.