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Immunoaffinity Columns IAC Combi Afla+Ochra+Zeara

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Multi-mycotoxin immunoaffinity columns for simultaneous extraction of Aflatoxin, Ochratoxin, and Zearalenone from complex matrices

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  • Triple mycotoxin extraction: Aflatoxins, Ochratoxins, and Zearalenones in one column
  • Wide dynamic range: 0.04–200 ng/column Aflatoxin + Ochratoxin; 5–800 ng/column Zearalenone
  • Exceptionally low background: <0.04 ng (HPLC-FLD detection limit)
  • High recovery efficiency: 85–110%
  • Broad cross-reactivity profile: Covers all major mycotoxin congeners
  • 3 mL column volume optimized for standard sample preparation workflows

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Combi immunoaffinity columns (IAC) designed for rapid and efficient purification of three major mycotoxin groups in a single preparation step. These pre-packed columns deliver high specificity and selectivity for Aflatoxin (B1, B2, G1, G2, M1), Ochratoxin A & B, and Zearalenone derivatives from food and feed samples prior to HPLC-FLD or LC-MS/MS analysis.

Technical Specifications

Working Range – Aflatoxin & Ochratoxin 0.04–200 ng per column
Working Range – Zearalenone 5–800 ng per column
Blank Contamination Level <0.04 ng (HPLC-FLD detection limit)
Recovery Rate 85–110%
Column Capacity – Aflatoxin 1.8 µg
Column Capacity – Ochratoxin 3 µg
Column Capacity – Zearalenone 2.5 µg
Cross-Reactivity – Aflatoxin B1 100%
Cross-Reactivity – Aflatoxin B2 86%
Cross-Reactivity – Aflatoxin G1 101%
Cross-Reactivity – Aflatoxin G2 82%
Cross-Reactivity – Aflatoxin M1 81%
Cross-Reactivity – Ochratoxin A 100%
Cross-Reactivity – Ochratoxin B 103%
Cross-Reactivity – Zearalenone 100%
Cross-Reactivity – α-Zearalanol 99%
Cross-Reactivity – β-Zearalanol 94%
Cross-Reactivity – α-Zearalenol 97%
Cross-Reactivity – β-Zearalenol 86%

Applications

  • HPLC-FLD analysis of mycotoxin residues in cereals, grains, and animal feed
  • LC-MS/MS confirmation testing for aflatoxin, ochratoxin, and zearalenone in food matrices
  • Regulatory compliance testing for official food and feed safety laboratories
  • Export commodity screening for international mycotoxin limits
  • Multi-residue sample preparation in contract testing laboratories
  • Pre-analytical cleanup for complex matrices (nuts, spices, dried fruits)

Frequently Asked Questions

Can this column be used for samples containing all three mycotoxin groups simultaneously?
Yes, the Combi design enables single-column extraction of Aflatoxins, Ochratoxins, and Zearalenones in parallel, reducing sample preparation time and solvent consumption.
What is the column volume and recommended sample loading?
The 3 mL column volume accommodates typical food and feed sample extracts. Column capacity limits should not be exceeded to maintain recovery efficiency (1.8 µg Aflatoxin, 3 µg Ochratoxin, 2.5 µg Zearalenone).
How should samples be prepared before passing through the column?
Sample preparation varies by matrix but typically involves extraction with aqueous or organic solvents followed by dilution and pH adjustment. Detailed protocols are provided in the technical datasheet.
Is the column compatible with both HPLC-FLD and LC-MS/MS?
Yes, the high purification efficiency of the immunoaffinity column is compatible with both fluorescence detection (HPLC-FLD) and tandem mass spectrometry (LC-MS/MS) methods.