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ClearBand 10x Tris Buffered Saline (TBS), pH 7.6

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10x TBS buffer solution formulated for western blotting and ELISA washing steps, pH 7.6. Dilute to 1x prior to use.

  • 10x concentrated TBS stabilized at pH 7.6
  • Suitable for western blotting and ELISA washing steps
  • Prepared with ultrapure water
  • Filter sterilized
  • Dilute to 1x working solution before use

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ClearBand 10x Tris Buffered Saline (TBS) is a salt solution stabilized at pH 7.6, specifically designed for washing steps in western blotting and ELISA procedures. It effectively removes unbound materials from membranes or plates while preserving antibody-antigen binding interactions.

The solution is prepared using ultrapure water and filter sterilized, ensuring reduced contamination risk and consistent, reproducible results. Supplied in 10x concentrated format, users can dilute to 1x working solution as needed, enabling prolonged stock usage with cost efficiency.

This product serves as a standard buffer for western blot washing steps or ELISA protocols. It is typically converted to TBST format by adding detergents such as Tween-20 (added by user; not included with product).

Technical Specifications

Product Type 10x Tris Buffered Saline (TBS)
pH 7.6
Concentration 10x (must be diluted to 1x prior to use)
Preparation Prepared with ultrapure water, filter sterilized
Manufacturer ClearBand
SKU TB05

Applications

  • Western blot membrane washing steps
  • ELISA plate washing procedures
  • Base buffer for TBST preparation (with addition of Tween-20)

Frequently Asked Questions

How should 10x TBS be diluted?
Prior to use, dilute with ultrapure water to 1x working concentration.
Can this buffer be used to prepare TBST?
Yes, after dilution to 1x, TBST can be prepared by adding Tween-20; Tween-20 is not included with this product.
Is the product sterile?
Yes, it has undergone filter sterilization.