Beta-Lactoglobulin ELISA kiti SENSISpec 96 kuyucuk

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Beta-Lactoglobulin ELISA Kit SENSISpec 96 Wells – Allergen Detection

Fiyat Sorunuz

High-sensitivity sandwich ELISA kit for quantitative detection of bovine milk residues in food products

Ultra-sensitive detection: 1.5 ppb limit of detection (LOD)
Quantitative range: 10 - 400 ppb
Sandwich enzyme immunoassay format for specificity
Optimized incubation protocol: 60 minutes total (20/20/20 minutes)
Microtiter plate format for high-throughput screening
Validated on BoltTM automated ELISA system
Excellent specificity: <0.2% cross-reactivity with sheep milk, <0.002% with goat milk
Extended shelf life: 13 months at 2–8°C

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Daha fazla detay

The SENSISpec β-Lactoglobulin ELISA kit represents a highly sensitive detection system specifically engineered for identification and quantification of bovine milk residues in cookies, cereals, sausages, orange juice, wine, soy products, and chocolate. This assay has been validated on the BoltTM automated ELISA system. Bovine milk protein remains one of the most significant allergenic food components, particularly for pediatric populations. Even trace amounts of bovine milk can trigger allergic reactions and may result in anaphylactic shock in severe cases. The presence of hidden milk proteins in processed meats, baked goods, ready-to-eat meals, and beverages poses critical challenges for milk-allergic individuals. Whey proteins comprise approximately 20% of total bovine milk proteins, with β-lactoglobulin as the predominant whey protein fraction—the primary immunoreactive component in milk allergy sensitization.

Technical Specifications

SKU	HU0030002
Test Principle	Sandwich enzyme immunoassay
Format	Microtiter plate
Incubation Time	60 minutes (20/20/20 minutes)
Standards	0; 10, 40; 100; 400 ppb
Detection Wavelength	450 nm (HRP + TMB)
Limit of Detection (LOD)	1.5 ppb
Quantitative Range	10 – 400 ppb
Cross-reactivity: Sheep Milk	< 0.2%
Cross-reactivity: Goat Milk	< 0.002%
Cross-reactivity: Casein	< 0.02%
Shelf Life	13 months
Storage Temperature	2–8°C

Applications

Detection of bovine milk residues in processed meat products and sausages
Verification of milk-free status in baked goods and confectionery products
Screening of allergen cross-contamination in cereal and grain-based foods
Quality assurance testing in juice and beverage manufacturing
Regulatory compliance testing for food labeling and allergen declarations
Validation of cleaning procedures in multi-use food processing facilities
Research applications in food allergy immunology and protein characterization

Frequently Asked Questions

What is the difference between this kit and other β-lactoglobulin assays?

The SENSISpec kit combines ultra-low detection limits (1.5 ppb) with validated automation compatibility on BoltTM systems, providing superior sensitivity and throughput for routine food safety testing compared to conventional manual ELISA methods.

Why is β-lactoglobulin monitored rather than total milk protein?

β-lactoglobulin is the primary whey protein fraction (~20% of bovine milk) and the dominant immunoreactive component in IgE-mediated milk allergy. Its quantification directly correlates with allergenic potential and is the industry standard allergen marker.

Is this kit suitable for detecting milk in processed products?

Yes. The assay has been specifically validated for cookies, cereals, processed meats, citrus juices, wine, soy-based products, and chocolate—accommodating complex food matrices with high specificity.

Can this assay differentiate between bovine and other mammalian milk sources?

Yes. Cross-reactivity testing shows minimal interference: <0.2% with sheep milk and <0.002% with goat milk, enabling source-specific allergen identification.