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PCR Purification Kit (Eco Spin) – EcoPP-50x, 50 Reactions

Fiyat Sorunuz

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Spin column kit designed for rapid and efficient purification of PCR products from primer dimers, free nucleotides, salts, and Taq polymerase. Also suitable for use after restriction digestion, alkaline phosphatase, and kinase reactions. A practical solution for molecular biology laboratories.

  • 50-reaction kit capacity (EcoPP-50x)
  • Removes primer dimers, free nucleotides, salts, and Taq polymerase
  • Spin column-based, rapid purification procedure
  • Suitable for restriction digestion, alkaline phosphatase, and kinase reactions
  • Compatible with standard centrifuge; no additional equipment required

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The Eco Spin PCR Purification Kit has been developed for molecular biology laboratories seeking to accelerate the post-PCR cleanup step. The kit effectively removes primer dimers, free nucleotides, salts, and Taq polymerase from the reaction mixture, yielding clean DNA for downstream analysis steps (sequencing, cloning, restriction digestion).

Beyond PCR products, the kit can be utilized for nucleic acid purification following enzymatic processes such as restriction enzyme digestion, alkaline phosphatase treatment, and kinase reactions. This versatility makes it a preferred choice for multiple applications within routine laboratory workflows.

Due to its spin column-based format, the procedure is straightforward and rapid, requiring no specialized equipment; it operates with a standard laboratory centrifuge. The EcoPP-50x designation indicates that the kit is packaged for 50 reactions.

Technical Specifications

Product Type PCR Purification Kit (Spin Column)
Kit Code EcoPP-50x
Capacity 50 reactions
Removed Components Primer dimers, free nucleotides, salts, Taq polymerase
Additional Applications Restriction digestion, alkaline phosphatase treatment, kinase reaction purification

Applications

  • DNA cleanup prior to sequencing or cloning after PCR
  • Purification following restriction enzyme digestion
  • Nucleic acid cleanup after alkaline phosphatase or kinase reactions

Frequently Asked Questions

What products can this kit purify?
The kit purifies PCR products as well as nucleic acids obtained from restriction digestion, alkaline phosphatase treatment, and kinase reactions.
How many reactions does this kit provide?
The EcoPP-50x designation indicates the kit is designed for 50 reactions.
Do I need specialized equipment?
The kit uses a spin column format and operates with a standard laboratory centrifuge.