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SENSISpec ELISA Crustaceans (Tropomyosin) Allergen 96 Wells

Fiyat Sorunuz

Highly sensitive sandwich ELISA kit for detection of tropomyosin protein from crustaceans in food matrices

  • Sandwich enzyme immunoassay format for high specificity
  • Tropomyosin-specific detection across crustacean species
  • Low detection limits across multiple food matrices
  • Quantitation range 20-400 ppb
  • 96-well microtiter plate format
  • 24-month shelf life with standard 2-8°C storage

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Crustaceans represent an important food allergen group. In this context, tropomyosin is the most critical protein found across all common crustacean species. In cooked crustacean extracts, this protein comprises approximately 20% of total proteins. Cross-contamination frequently occurs as a consequence of production processes, explaining why crustacean residues cannot be excluded from food products in many cases. Sensitive detection systems are therefore essential for identifying crustacean residues in food materials. The SENSISpec Crustaceans (Tropomyosin) ELISA provides highly sensitive detection of tropomyosin (from Penaeus indicus) and is specifically employed for quantifying crustacean residues in fish products, soups, sauces, baked goods, and meat products.

Technical Specifications

SKU HU0030006
Test Principle Sandwich enzyme immunoassay
Format Microtiter plate
Incubation Time 60 min (20/20/20 min)
Standards 0; 20, 60; 200; 400 ppb
Detection 450 nm (HRP + TMB)
Detection Limit (LOD) – Soy sauce 1.7 ppb
Detection Limit (LOD) – Vegetable soup 3.6 ppb
Detection Limit (LOD) – Baked goods 0.9 ppb
Detection Limit (LOD) – Fish 8.5 ppb
Detection Limit (LOD) – Meat 10.3 ppb
Quantitation Range 20-400 ppb
Cross-reactivities No
Shelf Life 24 months
Storage Conditions 2-8°C

Applications

  • Detection of crustacean allergen residues in processed fish products
  • Monitoring crustacean contamination in soup and sauce formulations
  • Allergen verification in baked goods and meat products
  • Quality control in food manufacturing facilities
  • Compliance testing for allergen labeling regulations
  • Cross-contamination assessment in production environments

Frequently Asked Questions

What is the primary target protein detected by this ELISA kit?
The kit specifically detects tropomyosin, the most abundant protein in crustacean extracts, comprising approximately 20% of total proteins in cooked samples.
What is the detection principle used?
The assay employs a sandwich enzyme immunoassay format with HRP detection at 450 nm using TMB substrate.
How long are the incubation steps?
Total incubation time is 60 minutes, divided into three 20-minute intervals for optimal antibody-antigen binding.