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EcoPURE Total RNA Kit – Rapid RNA Extraction from Multiple Sample Types

Fiyat Sorunuz

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High-quality total RNA purification from blood, cultured cells, and tissues including small RNA recovery

Purifies total RNA from whole blood, cultured cells, frozen and fresh tissues
Efficient recovery of small RNAs and microRNAs
Chaotropic ion and silica membrane-based purification technology
Eliminates phenol-chloroform, hazardous chemicals, and expensive resins
Rapid protocol: completes in less than 10 minutes at room temperature
High-quality RNA suitable for multiple downstream applications

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Daha fazla detay

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Daha fazla detay

EcoPURE Total RNA Kit is engineered for simple and efficient purification of high-quality total RNA, including small RNAs such as microRNAs, from whole blood, cultured cells, and frozen or fresh tissue samples. Utilizing chaotropic ion chemistry and silica-based membrane technology, this kit eliminates the requirement for expensive resins, hazardous phenol-chloroform extraction, β-mercaptoethanol, or labor-intensive alcohol precipitation methods. The streamlined protocol completes in less than 10 minutes at room temperature, with purified RNA suitable for all standard downstream applications including cDNA synthesis, northern blotting, differential display, primer extension, and mRNA enrichment.

Technical Specifications

Sample Sources	Whole blood, cultured cells, frozen tissues, fresh tissues
RNA Species	Total RNA including microRNAs and small RNA molecules
Protocol Duration	<10 minutes at room temperature
Purification Technology	Chaotropic ion and silica-based membrane chromatography

Applications

cDNA library construction and synthesis
Northern blotting and RNA hybridization
Differential display and RT-PCR
Primer extension assays
mRNA enrichment and poly(A) selection
Gene expression profiling
RNA sequencing (RNA-seq) preparation
microRNA analysis and quantification

Frequently Asked Questions

What biological samples can be processed with EcoPURE Total RNA Kit?

The kit is optimized for RNA extraction from whole blood, cultured cells, and both frozen and fresh tissue specimens.

Will this kit recover microRNAs and other small RNA species?

Yes, the silica-based membrane technology efficiently captures total RNA including microRNAs and other small RNA molecules.

What is the total procedure time?

The standard purification protocol requires less than 10 minutes at room temperature, enabling efficient high-throughput processing.

Which downstream applications are compatible with EcoPURE-purified RNA?

Purified RNA is suitable for cDNA synthesis, northern analysis, differential display PCR, primer extension, mRNA selection, RNA-seq library preparation, and other molecular biology applications.

Does this method avoid toxic extraction chemicals?

Yes, EcoPURE eliminates phenol-chloroform extraction, β-mercaptoethanol, expensive chromatography resins, and prolonged alcohol precipitation, providing a safer, eco-conscious alternative.